葡萄籽原花青素对人皮肤鳞状细胞癌A431细胞增殖和凋亡的影响  

Effect of grape seed proanthocyanidin on proliferation and apoptosis of human skin squamous cell carcinoma A431 cells

作　　者：李晓民 梁韦巍 韩冬 刘爱芹 韩志强 LI Xiaomin;LIANG Weiwei;HAN Dong;LIU Aiqin;HAN Zhiqiang(Weifang Institute of Dermatology,Weifang 261061,China;Anqiu Women and Children s Hospital of Weifang City in Shandong Province,Weifang 262100,China)

机构地区：[1]山东省潍坊市皮肤病防治所,潍坊261061 [2]山东省潍坊市安丘市妇幼保健医院,潍坊262100

出　　处：《中国麻风皮肤病杂志》2021年第11期696-699,共4页China Journal of Leprosy and Skin Diseases

基　　金：潍坊市科学技术发展计划项目(编号:2020YX078);潍坊市卫健委科研项目计划(编号:WFWSJK-2020-129)。

摘　　要：目的:明确葡萄籽原花青素(GSP)对人皮肤鳞状细胞癌A431细胞增殖和凋亡的影响。方法:体外培养A431细胞,用不同浓度的GSP(5、10、20、40、80μg/mL)处理,采用MTT实验和克隆形成实验检测细胞活力和增殖能力,Western blot检测细胞通路PI3K/Akt/GSK-3β信号转导通路蛋白的表达。结果:随着GSP浓度增加,A431细胞活力及细胞克隆逐渐下降,细胞凋亡逐渐增加。GSP处理后A431细胞中p-PI3K、p-Akt和p-GSK-3β表达水平明显降低。结论:GSP可以诱导A431细胞凋亡和抑制A431细胞增殖,其机制可能与抑制PI3K/Akt/GSK-3β信号通路活化有关。Objective:To determine the effect of grape seed proanthocyanidins(GSP)on proliferation and apoptosis of human skin squamous cell carcinoma A431 cells.Methods:A431 cells were cultured in vitro and treated with different concentrations of GSP(5,10,20,40,80μg/mL).The cell viability and proliferation were detected by MTT assay and colony formation assay respectively.The protein level of PI3K/Akt/GSK-3βsignal transduction pathway was detected by Western blot.Results:As the increasing of GSP concentration,the cell viability and cell clone level of A431 decreased gradually,apoptosis rate increased.The protein level of p-PI3K,p-Akt,p-GSK-3βdecreased after treated with GSP.Conclusion:GSP can induced apoptosis and inhabited proliferation of A431 cells,which may be related with the activation of PI3K/Akt/GSK-3βsignaling pathway.

关 键 词：葡萄籽原花青素 人皮肤鳞状细胞癌细胞 增殖 凋亡 

分 类 号：R285[医药卫生—中药学]