High expression of BCL6 inhibits the differentiation and development of hematopoietic stem cells and affects the growth and development of chickens  

作　　者：Hongmei Li Bowen Hu Shang Hu Wen Luo Donglei Sun Minmin Yang Zhiying Liao Haohui Wei Changbin Zhao Dajian Li Meiqing Shi Qingbin Luo Dexiang Zhang Qinghua Nie Xiquan Zhang 

机构地区：[1]Department of Animal Genetics,Breeding and Reproduction,College of Animal Science,South China Agricultural University,Guangzhou 510642,Guangdong Province,China [2]Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding and Key Lab of Chicken Genetics,Breeding and Reproduction,Ministry of Agriculture,Guangzhou 510642,Guangdong,China [3]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,Guangdong,China [4]Division of Immunology,Virginia-Maryland College of Veterinary Medicine,University of Maryland,College Park,MD,USA

出　　处：《Journal of Animal Science and Biotechnology》2021年第3期904-916,共13页畜牧与生物技术杂志（英文版）

基　　金：This work was supported by grants from the Key-Area Research and Development Program of Guangdong Province(Grant No.2020B020222002);the Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Livestock and Poultry,National Natural Science Foundation of China(Grant No.31401046);the China Agriculture Research System(CARS-41-G03);Guangdong Youth Talent Project.

摘　　要：Background:B-cell CLL/lymphoma 6(BCL6)is a transcriptional master regulator that represses more than 1200 potential target genes.Our previous study showed that a decline in blood production in runting and stunting syndrome(RSS)affected sex-linked dwarf(SLD)chickens compared to SLD chickens.However,the association between BCL6 gene and hematopoietic function remains unknown in chickens.Methods:In this study,we used RSS affected SLD(RSS-SLD)chickens,SLD chickens and normal chickens as research object and overexpression of BCL6 in hematopoietic stem cells(HSCs),to investigate the effect of the BCL6 on differentiation and development of HSCs.Results:The results showed that comparison of RSS-SLD chickens with SLD chickens,the BCL6 was highly expressed in RSS-SLD chickens bone marrow.The bone marrow of RSS-SLD chickens was exhausted and red bone marrow was largely replaced by yellow bone marrow,bone density was reduced,and the levels of immature erythrocytes in peripheral blood were increased.At the same time,the hematopoietic function of HSCs decreased in RSS-SLD chickens,which was manifested by a decrease in the hematopoietic growth factors(HGFs)EPO,SCF,TPO,and IL-3,as well as hemoglobinα1 and hemoglobinβexpression.Moreover,mitochondrial function in the HSCs of RSS-SLD chickens was damaged,including an increase in ROS production,decrease in ATP concentration,and decrease in mitochondrial membrane potential(ΔΨm).The same results were also observed in SLD chickens compared with normal chickens;however,the symptoms were more serious in RSS-SLD chickens.Additionally,after overexpression of the BCL6 in primary HSCs,the secretion of HGFs(EPO,SCF,TPO and IL-3)was inhibited and the expression of hemoglobinα1 and hemoglobinβwas decreased.However,cell proliferation was accelerated,apoptosis was inhibited,and the HSCs entered a cancerous state.The function of mitochondria was also abnormal,ROS production was decreased,and ATP concentration andΔΨm were increased,which was related to the inhibition of apoptosis of stem ce

关 键 词：BCL6 Hematopoietic stem cells Mitochondrial function Runting and stunting syndrome Sex-linked dwarf chickens 

分 类 号：S831[农业科学—畜牧学]