兴安升麻的环阿尔廷类三萜成分及其生物活性研究  

Study on the cycloartane triterpenoids from Cimicifuga dahurica and their biological activity

作　　者：李谭谭 孟庆红 花尔并[1] 孙奕[2] LI Tan-tan;MENG Qing-hong;HUA Er-bing;SUN Yi(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Institute of Chinese Materia Medica.China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国药物化学杂志》2021年第7期520-531,共12页Chinese Journal of Medicinal Chemistry

基　　金：国家科技重大专项课题(2014ZX09509-001001007)。

摘　　要：目的对兴安升麻的化学成分及其生物活性进行研究。方法运用正、反相硅胶柱色谱、薄层色谱、凝胶柱色谱、高效液相色谱等分离纯化手段以及LC-MS技术与活性跟踪分离方法进行分离,并根据化合物的理化性质和波谱数据阐明化学结构。采用MTT法测定所分离化合物的活性。结果与结论从升麻的乙酸乙酯提取物中分离得到22个三萜类化合物,分别鉴定为:槭树醇(1)、24-表-槭树醇(2)、大三叶升麻醇(3)、7,8-去氢升麻醇(4)、24-表-7,8-去氢升麻醇(5)、cimiricaside A(6)、24-O-乙酰-7,8-二去氢升麻烷-3-O-β-D-木糖苷(7)、(23R,24R)-16β,23,16α,24-二环氧-12β-乙酰氧基-环烷-7-en-3,15,25-三醇3-O-β-D-木糖苷(8)、7,8-去氢升麻醇-3-O-β-D-木糖苷(9)、24-O-乙酰基-7,8-二去氢升麻烷-3-O-β-D-木糖苷(10)、cimiricaside E(11)、24-表-24-O-乙酰基-7,8-二去氢升麻烷3-O-β-D-木糖苷(12)、7,8-去氢升麻醇3-O-α-L-阿拉伯糖苷(13)、24-表-24-O-乙酰-7,8-二去氢升麻烷-3-O-D-半乳糖苷(14)、25-O-乙酰基-7,8-二去氢升麻醇3-O-β-D-木糖苷(15)、24-O-乙酰基-25-O-甲基-7,8-二去氢升麻烷-3-O-β-D-木糖苷(16)、12β-羟基-7(8)-en-升麻醇(17)、cimiracemosideⅠ(18)、25-O-乙酰升麻醇-3-O-β-D-木糖苷(19)、7,8-去氢-24-乙酰羟基升麻烷-3-O-α-L-阿拉伯糖苷(20)、7,8-二去氢-25-脱氢升麻醇-3-O-β-D-木糖苷(21)、cimiricaside B(22)。其中化合物13、14、17、18、20均为首次从该植物中分离得到,化合物2、3、8、12具有较好的细胞毒活性。Cimicifuga foetida is a commonly used traditional Chinese medicine,w idely distributed in Europe,North America,and Asia.In this paper,tw enty tw o bioactive cycloartane triterpenoids w ere isolated by using various column chromatography,TLC,HPLC,as w ell as LC-M S technologies.The isolated cycloartane triterpenoids w ere identified by the physical and chemical properties and spectroscopic analysis as acerinol(1),24-epi-acerinol(2),heracleifolinol(3),7,8-didehydrocimigenol(4),24-epi-7,8-didehydrocimigenol(5),cimiricaside A (6),24-O-acetyl-7,8-didehydrohydroshengmanol 3-O-β-D-xylopyranoside(7),(23R,24R)-16β,23,16a,24-diepoxy-12β-acetoxy-cycloart-7-en-3β,15a,25-triol 3-O-β-D-xylopyranoside (8),7,8-didehydrocimigenol 3-O-β-D-xylopyranoside(9),24-epi-7,8-didehydrocimigenol 3-O-β-D-xylopyranoside(10),cimiricaaside E(11),24-epi-24-O-acetyl-7,8-didehydroshengmanol 3-O-β-D-xylopyranoside(12),7,8-didehydrocimigenol 3-O-α-L-arabinopyranoside (13),24-epi-24-O-acetyl-7,8-didehydroshengmanol-3-O-D-galactopyranoside(14),25-O-acetyl-7,8-didehrocimigenol-3-O-β-D-xylopyranoside(15),24-O-acetyl-25-O-methyl-7,8-didehydrohydroshengmanol-3-O-β-D-xylopyranoside (16),12β-hydroxy-7 (8)-en-cimigenol(17),cimiracemosideⅠ(18),25-O-acetyl-cimigenol-3-O-D-xylopyranside (19),7,8-didehydro-24-acetyldroshengmanol-3-O-α-L-arabinopyranoside (20),7,8-didehydro-25-dehydrocimigenol-3-O-β-D-xylopyranoside(21),cimiricaside B (22).Compounds 13,14,17,18,and 20 w ere isolated from the C.dahurica for the first time.

关 键 词：兴安升麻 环阿尔廷类三萜 生物活性 结构鉴定 

分 类 号：R284[医药卫生—中药学]