乌珠穆沁羊DGAT1、NR6A1、MAN1A1基因多态性与其生长性状的关联分析  

Association Analysis Between Polymorphisms of DGAT1,NR6A1 and MAN1A1 Genes and Growth Traits in Ujumqin Sheep

作　　者：张晋华 尚明玉 胡文萍[1] 张莉[1] ZHANG Jinhua;SHANG Mingyu;HU Wenping;ZHANG Li(Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国畜牧兽医》2021年第9期3312-3322,共11页China Animal Husbandry & Veterinary Medicine

基　　金：内蒙古自治区科技重大专项(2020ZD0003);农业农村部畜禽种质资源保护项目(19200531);农业农村部农业品种改良提升专项经费。

摘　　要：试验旨在探究甘油二酯酰基转移酶(diacylglycerol acyltransferase 1,DGAT1)基因g.13504098 C>T位点、核受体辅抑制子1(nuclear receptor subfamily 6 group A member 1,NR6A1)基因g.11204707 A>C位点和α-甘露糖苷酶(alpha-mannosidase,MAN1A1)基因g.18795097 C>T位点的多态性与乌珠穆沁羊生长性状之间的关系,为高产乌珠穆沁羊分子选育提供新的遗传标记。运用Sequenom MassARRAY®SNP技术对乌珠穆沁羊DGAT1、NR6A1和MAN1A1基因的3个多态位点g.13504098 C>T、g.11204707 A>C和g.18795097 C>T进行检测,同时利用线性混合模型分析基因型与其4、6月龄生长性状的关联性。结果显示,在这3个位点中均检测到3种基因型,具体为:g.13504098 C>T位点的基因型为:CC、CT和TT,优势基因型为CC(0.49),优势等位基因为C(0.70);g.11204707 A>C位点的基因型为:AA、CA和CC,优势基因型为AA(0.54),优势等位基因为A(0.73);g.18795097 C>T位点的基因型为:CC、TC和TT,优势基因型为TC(0.51),优势等位基因为C(0.55)。卡方检验显示,g.13504098 C>T、g.11204707 A>C和g.18795097 C>T位点在乌珠穆沁羊群体中均处于哈代-温伯格(Hardy-Weinberg)平衡状态(P>0.05)。群体遗传学分析表明,这3个位点在乌珠穆沁羊种群中属于中度多态性(0.25<PIC<0.50)。关联分析结果显示,g.13504098 C>T位点与乌珠穆沁羊4、6月龄的体重、体高、胸围、管围及6月龄的胸宽均呈极显著相关(P<0.01);g.11204707 A>C位点与乌珠穆沁羊4、6月龄的体高、体斜长及4月龄的体重、6月龄的胸围均呈极显著相关(P<0.01),与6月龄的体重、管围均呈显著相关(P<0.05);g.18795097 C>T位点与乌珠穆沁羊4月龄的体高、6月龄的胸围均呈极显著相关(P<0.01),与4月龄的体重、胸围以及6月龄的胸宽均呈显著相关(P<0.05)。综上所述,DGAT1基因g.13504098 C>T、NR6A1基因g.11204707 A>C和MAN1A1基因g.18795097 C>T位点多态性与乌珠穆沁羊群体部分生长性状具有显著的关联性,可以作为乌珠穆�The aim of this study was to elucidate the polymorphisms of g.13504098 C>T of diacylglycerol acyltransferase 1(DGAT1)gene,g.11204707 A>C of nuclear receptor subfamily 6 group A member 1(NR6A1)gene and g.18795097 C>T of alpha-mannosidase(MAN1A1)gene,and its association with the growth traits in Ujumqin sheep,providing new genetic markers for high productivity molecular breeding of Ujumqin sheep.g.13504098 C>T,g.11204707 A>C and g.18795097 C>T of DGAT1,NR6A1 and MAN1A1 genes in Ujumqin sheep were detected by Sequenom MassARRAY®SNP assay,and the linear mixed model was used to analyze the association between genotypes and the growth trait in Ujumqin sheep at 4 and 6 months of age.The results showed that three genotypes were detected at three loci.Specifically,g.13504098 C>T included CC,CT and TT genotypes,CC genotype was dominant genotype(0.49),C was the dominant allele(0.70).g.11204707 A>C included AA,CA and CC genotypes,AA genotype was dominant genotype(0.54),A was the dominant allele(0.73).g.18795097 C>T included CC,TC and TT genotypes,TC genotype was dominant genotype(0.51),C was the dominant allele(0.55).χ^(2) test indicated that g.13504098 C>T,g.11204707 A>C and g.18795097 C>T of Ujumqin sheep were in Hardy-Weinberg equilibrium(P>0.05).Population genetic analysis showed that g.13504098 C>T,g.11204707 A>C and g.18795097 C>T were at moderate polymorphisms in Ujumqin sheep population(0.25<PIC<0.50).Association analysis revealed that g.13504098 C>T was extremely significantly associated with the body weight,body height,chest girth,shin circumference of Ujumqin sheep at 4 and 6 months of age,and the chest width at 6 months of age(P<0.01);g.11204707 A>C was extremely significantly associated with the body height and body length of Ujumqin sheep at 4 and 6 months of age,the body weight at 4 months of age,and the chest girth at 6 months of age(P<0.01),and it was significantly associated with the body weight and shin circumference at 6 months of age(P<0.05);g.18795097 C>T was extremely significantly associated with th

关 键 词：绵羊 DGAT1基因 NR6A1基因 MAN1A1基因 生长性状 

分 类 号：S813.3[农业科学—畜牧学]