活性炭对鹅膏毒肽及鬼笔毒肽的体外吸附研究  

In vitro adsorption of amatoxin and phallotoxin by activated carbon

作　　者：钟加菊 姚群梅 唐燕 蒲艳 余成敏 Zhong Jia-ju;Yao Qun-mei;Tang Yan;Pu Yan;Yu Cheng-min(The People's Hospital of Chuxiong Yi Autonomous Prefecture,Chuxiong 675000,China)

机构地区：[1]楚雄彝族自治州人民医院,云南楚雄675000

出　　处：《中国急救医学》2021年第8期709-713,共5页Chinese Journal of Critical Care Medicine

基　　金：云南省教育厅科学研究基金项目(2020J0609);云南省科技计划项目重大科技专项(2018ZF009);云南省地方高校联合专项(202001BA070001-046)。

摘　　要：目的研究活性炭对致命鹅膏中α-鹅膏毒肽(α-amanitin,α-AMA)及β-鹅膏毒肽(β-amanitin,β-AMA)、羧基三羟鬼笔毒肽(phallisacin,PSC)、羧基二羟鬼笔毒肽(phallacidin,PCD)的吸附效果。方法应用反相高效液相色谱法检测不同吸附剂、不同时间、不同剂量及不同p H条件下对致命鹅膏中α-AMA、β-AMA、PSC及PCD吸附情况。结果 (1)活性炭粉对α-AMA、β-AMA、PSC及PCD的吸附率远高于药用炭片、蒙脱石散及白陶土(P <0.01)。毒素吸附总量符合活性炭粉>药用炭片>蒙脱石散>白陶土,p H 6.8时分别为6.36 mg/g、0.85mg/g、0.84 mg/g及0.48 mg/g;p H 1.2时分别为5.87 mg/g、1.29 mg/g、1.11 mg/g及0.18 mg/g。(2)活性炭粉对毒素吸附在1 h内达到快速平衡,p H 6.8时α-AMA、β-AMA、PSC及PCD的吸附率分别为56.56%、26.40%、54.78%及51.93%;p H 1.2时分别为35.70%、52.82%、59.55%及62.39%。(3)活性炭粉对毒素的吸附呈现剂量依赖,当剂量为300.00 mg时,对所有毒素的吸附率均> 85.00%。(4)毒素吸附总量在p H 1.2、p H 2.0、p H 5.0、p H 6.8和p H 8.0分别为5.87 mg/g、5.65 mg/g、4.68 mg/g、6.23 mg/g和5.99 mg/g,p H 5.0时毒素吸附总量显著低于其他p H(P <0.01)。结论活性炭粉对致命鹅膏中鹅膏毒肽和鬼笔毒肽吸附效果最好,其吸附呈现时间和剂量依赖性,并且在胃肠道环境中呈现很好的吸附效果。Objective To investigate the adsorption efficiency of α-amanitin( α-AMA),β-amanitin( β-AMA),phallisacin( PSC),and phallacidin( PCD) by activated carbon. Methods Reverse phase high-performance liquid chromatography was used to analyze the adsorption condition ofα-AMA,β-AMA,PSC,and PCD in different adsorbent,time,dosage and p H. Results(1)The adsorption efficiency of α-AMA,β-AMA,PSC,and PCD by activated carbon powder was significantly higher than medicinal charcoal tablets,diosmectite and bolus alba( P < 0. 01). The total amount of toxin adsorption was consistent with activated carbon powder > medicinal charcoal tablets > diosmectite > bolus alba with 6. 36 mg/g,0. 85 mg/g,0. 84 mg/g,0. 48 mg/g in p H 6. 8,and 5. 87 mg/g,1. 29 mg/g,1. 11 mg/g,0. 18 mg/g in p H 1. 2 respectively.(2)The adsorption of toxins by activated carbon powder reached a rapid equilibrium within 1 hour,and the adsorption efficiency of α-AMA,β-AMA,PSC,and PCD was 56. 56%,26. 40%,54. 78%,51. 93% in p H 6. 8,and 35. 70%,52. 82%,59. 55%,62. 39% in p H 1. 2.(3)The adsorption by activated carbon powder presented dose dependence,and the adsorption efficiency of four toxins was more than 85. 00% when the adsorbent was 300. 00 mg.(4)The total amount of toxin adsorption was 5. 87 mg/g,5. 65 mg/g,4. 68 mg/g,6. 23 mg/g,5. 99 mg/g in p H 1. 2,p H 2. 0,p H 5. 0,p H 6. 8 and p H 8. 0 respectively. The total amount of toxin adsorption was obviously lower in p H 5. 0 than in other p H condition( P < 0. 01). Conclusions The activated carbon powder had the best adsorption for the amatoxin and phallotoxin in Amanita exitialis,and the adsorption showed time and concentration dependence,good adsorption appears in the gastrointestinal environment.

关 键 词：活性炭 致命鹅膏 体外吸附 鹅膏毒肽 鬼笔毒肽 

分 类 号：R994.4[医药卫生—毒理学]