Raceway pond cultivation of a new Arthrospira sp.ZJWST-S1 in digested piggery wastewater treated by MBR and ozonation  

作　　者：Liu Rui Yu Qiangqiang Guo Qingqing Zheng Wei Wang Genrong Chen Lujun 

机构地区：[1]Zhejiang Provincial Key Laboratory of Water Science and Technology,Department of Environment in Yangtze Delta Region Institute of Tsinghua University,Jiaxing,Zhejiang 314006,China [2]Nanhu Agricultural Economical Bureau,Jiaxing,Zhejiang 314000,China [3]School of Environment,Tsinghua University,Beijing 100084,China

出　　处：《International Journal of Agricultural and Biological Engineering》2017年第1期115-124,共10页国际农业与生物工程学报（英文）

基　　金：Zhejiang Provincial Key Research Project(2014C03022).

摘　　要：A new Arthrospira strain named Arthrospira sp.ZJWST-S1 was isolated from a local digested piggery wastewater(DPW)storage pool in Jiaxing City,Zhejiang Province,China.It possessed good stain resistance against contaminants in DPW,which was pretreated with a membrane bioreactor(MBR).The strain was identified as Arthrospira platensis(A.platensis)based on its morphological characteristics and the 16S rDNA sequencing analysis.The effect of chrominance on growth of A.platensis ZJWST-S1 was investigated in a field raceway pond filled with MBR effluent or MBR effluent decolorized with ozonation.After ozonation,the chrominance of MBR effluent was decreased from 700 mg Pt/L to 150 mg Pt/L.Two runs of cultivation showed that A.platensis ZJWST-S1 grew faster in the ozone decolorized MBR effluent,the averaged biomass concentration being 0.907 g/L after 10 days of cultivation,close to that in a Zarrouk medium(0.969 g/L).By comparison,the biomass grew much slower in the non-decolorized MBR effluent(0.624 g/L).The pollutant removal was also benefited from the accelerative growth of A.platensis ZJWST-S1 in the decolorized MBR effluent.Almost all ammonium,61.2%of nitrate and 68.1%of phosphate were removed by the A.platensis ZJWST-S1 in the decolorized MBR effluent,much higher than the corresponding values of almost all ammonium,25.4%of nitrate and 36.5%of phosphate in the MBR effluent.Furthermore,the Arthrospira biomass harvested from the ozone decolorized MBR effluent after 10 d cultivation was with crude protein content of 59.1%±3.5%in dry algae powder.The content of Pb,As,Cd and Hg in biomass was also low enough to meet the Chinese Arthrospira Standard for Animal Feed(GB/T 17243-1998).This study showed that the new strain A.platensis ZJWST-S1 possessed potential to be used for producing animal feed and simultaneous removal of nitrogen and phosphorus in DPW.

关 键 词：digested piggery wastewater(DPW) ozone decolorization Arthrospira platensis 16S rDNA nitrogen and phosphorus recovery 

分 类 号：X70[环境科学与工程—环境工程]