兔抗肠道病毒71型血清制备中免疫剂量对抗体效价的影响  

Effects of immune doses in rabbit anti-enterovirus 71 serum preparation on the antibody titer

作　　者：张玉平 胡云光[1] 殷安国[1] 李亚东[1] 杜宋婷 王雯慧 李晓颖 常亚军[1] 段男 宋杰[1] Zhang Yuping;Hu Yunguang;Yin Anguo;Li Yadong;Du Songting;Wang Wenhui;Li Xiaoying;Chang Yajun;Duan Nan;Song Jie(Department of Quality Control,Institute of Medical Biology,Chinese Academy of Medical Sciences&Peking Union Medical College,Kunming 650118,China)

机构地区：[1]中国医学科学院,北京协和医学院,医学生物学研究所质量检定室,昆明650118

出　　处：《国际生物制品学杂志》2021年第4期186-189,共4页International Journal of Biologicals

基　　金：云南省基础研究计划面上项目(2018FB026)。

摘　　要：目的研究兔抗肠道病毒71型(enterovirus 71,EV71)血清制备中不同免疫剂量对抗体效价的影响。方法将9只实验兔简单随机分为3组,每组3只。5、50和100μg 3个剂量EV71灭活疫苗和弗氏佐剂均匀混合后,通过肌内注射途径分别免疫3组动物,于第0、7、14及28天各免疫1次,每7天采血,分离血清,检测抗EV71中和抗体效价。结果100μg剂量组兔血清抗体效价显著高于5和50μg剂量组(第42天t值分别为-3.282和-2.486,P值均<0.05)。第42天时,兔血清抗体效价和免疫剂量(5~100μg范围内)呈线性关系,回归方程y=478.229x-2041.820。结论在本实验选择的3个免疫剂量中,随着免疫剂量升高,产生的血清抗体效价也升高,且呈线性关系。Objective To study the effect of different immune doses in rabbit anti-enterovirus 71(EV71)serum preparation on the antibody titer.Methods Three doses of 5,50 and 100μg were selected in this experiment.There were 3 groups of 3 rabbits in each group.After the mixture of EV71 inactivated vaccine and Freund’s adjuvant,the rabbits in the three groups were immunized by intramuscular injection.The rabbits were immunized once at 0,7,14 and 28 d,respectively.Blood samples were collected every 7 d,serum was separated and neutralizing antibody titer was detected.Results The antibody titer of 100μg was significantly higher than that of 5μg(t=-3.282)and 50μg(t=-2.486),on day 42(P<0.05).There was a linear relationship between the titer of rabbit serum antibody and the immune dose(within the range of 5-100μg).The linear model was established and the regression equation y=478.229x-2041.820 was obtained.Conclusion Among the 3 immune doses selected in this experiment,the titer of serum antibody increases with the increase of immune dose,and shows a linear relationship.

关 键 词：免疫血清 剂量效应关系 免疫 肠道病毒71型 抗体效价 

分 类 号：R373.2[医药卫生—病原生物学] R392-33[医药卫生—基础医学]