HPLC法测定蒙药嘎鲁-10散中羟基红花黄色素A的含量  

HPLC determination of hydroxysafflor pigment A in Mongolian medicine preparation Galu-10 decoction

作　　者：吕颖 赵虎义 郭莘莘 曹月 LV Ying;ZHAO Hu-yi;GUShen-shen;CAO Yue(Chifeng Products Quality Inspection And Testing Center,Inner Mongolia Autonomous Region,Chifeng,024000)

机构地区：[1]赤峰市产品质量检验检测中心,内蒙古赤峰024000

出　　处：《中国民族医药杂志》2021年第8期35-36,共2页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：内蒙古蒙中药标准建设项目(编号:JYCZ18-027-6)。

摘　　要：目的:建立蒙药嘎鲁-10散中羟基红花黄色素A的含量测定方法。方法:色谱柱采用Diamonsil Plus(250 mm×4.6 mm,5μm),流动相:甲醇-乙腈-0.7%磷酸溶液(用三乙胺调节pH至6.0±0.1)(26∶2∶72);流速:1.0 m L/min;检测波长:403 nm;柱温:30℃。结果:羟基红花黄色素A在7.979-79.79μg/mL范围内与峰面积线性关系良好,回归方程为Y=3.260×10^(4)X+1.101×10^(3)(R2=0.99998),平均加样回收率为99.1%,RSD为0.63%(n=9)。结论:该方法科学、简便、专属性较强,可有效控制嘎鲁-10散丸中羟基红花黄色素A的质量。Objective:To develop the methods for the determination of hydroxysafflor pigment A in Mongolian medicine preparation Galu-10 decoction.Method:Column was Diamonsil Plus column(250 mm×4.6 mm,5 μm),and the mobile phase was methanol-acetonitrile-0.7% phosphoric acid solution(Adjust p H with triethylamine to 6.0±0.1)(26∶2∶72),with flow rate1 mL/min,detection wavelength 403 nm,the column temperature was 30℃. Results:The linear relationship between curcumin and peak area was good in the range of 7.979-79.79 μg/mL. The regression equation was Y=3.260 ×10^(4) X+1.101×10^(3)(R2 =0.99998),the average recovery was 99.1%,and the RSD was 0.63%(n =9).Conclusion:The method is scientific,simple and specific,and can effectively control the quality of piperine in Mongolian medicine preparation.

关 键 词：嘎鲁-10散 羟基红花黄色素A 高效液相色谱法 含量测定 

分 类 号：K291.2[历史地理—历史学]