两种DNA族群推断技术在一组家系样本中的比较  

The application of DNA ancestry inference technique for analysis of a family samples

作　　者：王庆国 江丽[2] 王文平 张涛[4] 谢何鑫 李唐松 马原 孙文平 韩俊萍[2,5] Wang Qingguo;Jiang Li;Wang Wenping;Zhang Tao;Xie Hexin;Li Tangsong;Ma Yuan;Sun Wenping;Han Junping(First Affiliated Hospital of Shandong First Medical University(Shandong Provincial Qianfoshan Hospital),Jinan 250000,China;Key Laboratory of Forensic Genetics,Beijing Engineering Research Center of Crime Scene Evidence Examination,National Engineering Laboratory for Forensic Science,Institute of Forensic Science,Beijing 100038,China;Xinjiang production and construction corps of the third division Tumushuke municipal Public Security Bureau,Tumushuke,844000,China;Xinjiang Production and Construction Corps public security bureau,Urumqi 833000,China;Technology Department of Chaoyang Sub-bureau,Beijing Public Security Bureau,Beijing,100025,China)

机构地区：[1]山东第一医科大学第一附属医院(山东省千佛山医院)病理科,山东济南250000 [2]公安部物证鉴定中心,法医遗传学公安部重点实验室,北京市现场物证检验工程技术研究中心,现场物证溯源技术国家工程实验室,北京100038 [3]新疆生产建设兵团第三师图木舒克市公安局,新疆图木舒克844000 [4]新疆生产建设兵团公安局,新疆乌鲁木齐833000 [5]北京市公安局朝阳分局,北京100025

出　　处：《中国法医学杂志》2021年第4期388-393,共6页Chinese Journal of Forensic Medicine

基　　金：2017国家重点研发计划(2017YFC0803501);国家自然基金课题(81772027);中央级公益性科研院所基本科研业务费专项资金计划(2017JB026);2018首都科技领军人才培养工程支持(Z18110006318006)。

摘　　要：目的使用课题组构建的27-plex SNPs和38-plex InDels两套族群推断体系,检测一组维族与回族通婚家系样本,通过两种体系间的相互印证,以获得更可靠的种族分析结果。方法分别利用两套检测体系,对来自一维族—回族通婚家系的三代成员共16份样本进行检测,推断其族源信息。结果 16份样本在两种体系下的推断结果与样本的实际来源信息基本一致,该家族成员的遗传成分呈现出欧亚混合特征。结论本实验室构建的两种不同遗传标记的族群推断体系可以有效区分洲际人群的祖先来源,通过两种体系的相互印证,可为案件的侦查提供更为准确的科学线索和依据,同时38-plex InDels体系具有操作步骤简单,检测时间短,可以直接扩增等优势。Objective To discuss reliable information on ethnic sources using the previously constructed 27-plex SNPs and 38-plex InDels ancestry inference system that have been applied to detect and analyze the genetic information of a family sample of 16 individuals. Methods 16 family samples were tested using the 27-plex SNPs and 38-plex InDels ancestry inference systems to predict the ancestral region. Results All of the prediction results from two systems are in concordance with the known ancestry of these samples, and the genetic components of the family members show mixed Eurasian characteristics. Conclusion The results demonstrated that both of the 27-plex SNPs and 38-plex InDels ancestry inference systems can be effectively used to analyze the genetic components and ancestral origin of a unknown DNA sample. The mutual corroboration of the two systems can provide more accurate scientific clues for the investigation of cases. Compared to 27-plex SNPs, 38-plex In Dels exhibit the advantages of simplicity and convenience for operation, short time consuming, and stable direct amplification.

关 键 词：法医遗传学 族群推断 27-plex SNPs族群推断体系 38-plex InDels族群推断体系 

分 类 号：DF795.1[医药卫生—法医学]