Phase-feeding strategy for Chlorella vulgaris to enhance biomass and lipid productivity  

作　　者：Chang Haixing Liao Qiang Fu Qian Huang Yun Xia Ao Zheng Yaping Sun Yahui Zhu Xun 

机构地区：[1]Key Laboratory of Low-grade Energy Utilization Technologies and Systems,Chongqing University,Ministry of Education,Chongqing 400030,China [2]Institute of Engineering Thermophysics,Chongqing University,Chongqing 400030,China

出　　处：《International Journal of Agricultural and Biological Engineering》2017年第2期205-215,共11页国际农业与生物工程学报（英文）

基　　金：the State Key Program of National Natural Science of China(No.51136007);the International Cooperation and Exchange of the National Natural Science Foundation of China(No.51561145013);the National Science Foundation for Young Scientists of China(No.51606020);the National Key Research and Development Program,China(2016YFB0601002);the Postdoctoral Scientific Research Project of Chongqing,China(Xm2015070);the National Natural Science Funds for Young Scholar(51506017).

摘　　要：A phase-feeding strategy of nutrients based on requirements of Chlorella vulgaris in different physiological phases was examined to maximize the biomass and lipid productivity.This strategy includes reduction of duration in adaption phase(stage-I),enhancement of biomass in growth phase(stage-II)and improvement of lipid productivity in stationary phase(stage-III).The duration of microalgae in adaption phase was reduced from 52 h to 34 h at nitrogen and phosphorus feeding rates of 5.11 mg/(L·d)and 0.54 mg/(L·d),whereas the maximum biomass concentration during growth phase was improved to(4.03±0.25)g/L at nitrogen and phosphorus feeding rates of 20.04 mg/(L·d)and 4.21 mg/(L·d).In stationary and decline phases,a maximum lipid productivity of 132.30 mg/(L·d)was achieved when nutrients supply was stopped at 128 h,which was 28.86 mg/(L·d)higher than that when nutrients supply was stopped at 104 h.This multi-phase cultivation could be a promising strategy for simultaneous enhancement of microalgae biomass and lipid productivity.

关 键 词：cultivation strategy lipid productivity MICROALGAE nutrients regulation 

分 类 号：TG1[金属学及工艺—金属学]