木犀草素对兔耳增生性瘢痕抑制作用机制的初步研究  

Preliminary Study on the Inhibitory Mechanism of Luteolin on Hypertrophic Scar in Rabbit Ear

作　　者：李泰平[1] 肖红[1] LI Tai-ping;XIAO Hong(Neuro-Psychiatric Institute,Affiliated Brain Hospital,Nanjing Medical University,Nanjing 210029,Jiangsu,China)

机构地区：[1]南京医科大学附属脑科医院研究所,江苏南京210029

出　　处：《中国美容医学》2021年第8期91-94,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:初步研究木犀草素对兔耳增生性瘢痕的抑制作用及可能机制。方法:9只新西兰兔随机分为空白组、生理盐水对照组和药物干预组,每组3只,生理盐水对照组和药物干预组建立兔耳增生性瘢痕模型,每只兔左右耳各3个直径约0.7cm瘢痕创面,相距约1cm。生理盐水对照组给予生理盐水,药物干预组给予木犀草素乳膏,瘢痕组织于给药前和给药后40d取材,Masson染色后显微镜下观察各组瘢痕组织的病理变化,ELISA法检测瘢痕组织中转化生长因子-β(TGF-β)、血小板衍化生长因子(PDGF-BB)、结缔组织生长因子(CTGF)的含量,qRT-PCR和Western Blot法检测TGF-β、Ⅰ型胶原蛋白(CollagenⅠ)和基质金属蛋白酶-2(MMP-2)的表达水平。结果:Masson染色显示木犀草素干预后瘢痕组织中胶原沉积减少,胶原纤维致密且排列规则。与生理盐水对照组相比,药物干预组瘢痕组织中TGF-β、PDGF-BB、CTGF的表达明显减少,差异有统计学意义(P<0.05)。此外,qRT-PCR和Western Blot结果显示,与生理盐水对照组相比,药物干预组MMP-2表达升高,而CollagenⅠ表达水平明显降低,差异有统计学意义(P<0.05)。结论:木犀草素能够抑制兔耳增生性瘢痕的形成,其作用机制可能与抑制TGF-β、PDGF-BB、CTGF的分泌、上调MMP-2、减少CollagenⅠ的表达有关。Objective To reveal the inhibitory effect of luteolin and its mechanism on hypertrophic scar formation in rabbit ears.Methods 9 New Zealand rabbits were randomly divided into 3 groups:the blank group,the normal saline control group and the drug intervention group,with 3 rabbits in each group.Hypertrophic scar model was established in rabbit ears in the normal saline control group and the drug intervention group.Before and forty days after treatment,the pathological changes of the scar tissues were examined,and the collagen fibers hyperplasia were observed with Masson staining.The expression levels of TGF-β,PDGF-BB,CTGF were quantified by ELISA.In addition,TGF-β,CollagenⅠand MMP-2 were separately detected by quantitative real-time PCR(qRT-PCR)assay and Western Blot.Results Masson staining showed that collagen deposition was significantly reduced after luteolin treatment,collagen fibers were also dense and arranged regularly.ELISA results indicated that the expression levels of TGF-β,PDGF-BB and CTGF were obviously reduced in the drug intervention group compared to the the normal saline control group,the differences were statistically significant(P<0.05).In addition,the results of qRT-PCR and Western blot showed that compared with the normal saline control group,the expression of MMP-2 in the drug intervention group increased,while the expression level of CollagenⅠdecreased significantly(P<0.05).Conclusion Luteolin could inhibit the formation of hypertrophic scar in rabbit ears,and its mechanism may be related to the inhibition of TGF-β、the secretion of PDGF-BB and CTGF was related to the up regulation of MMP-2 and the decrease of collagenⅠexpression in vivo.

关 键 词：木犀草素 增生性瘢痕 兔耳模型 机制研究 

分 类 号：R619.6[医药卫生—外科学]