Photosynthetic profiling of a Dunaliella salina mutant DS240G-1 with improved β-carotene productivity induced by heavy ions irradiation  

作　　者：Yimei Xi Ying Liu Zhanyou Chi Liang Yin Lijuan Wang Guanghong Luo 

机构地区：[1]Gansu Engineering Technology Research Center for Microalgae,Hexi University,Zhangye 734000,Gansu,China [2]State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology,Shenzhen Academy of Environmental Science,Shenzhen 518001,Guangdong,China [3]School of Bioengineering,Dalian University of Technology,Dalian 116024,Liaoning,China

出　　处：《International Journal of Agricultural and Biological Engineering》2021年第2期211-217,共7页国际农业与生物工程学报（英文）

基　　金：This work was supported by the National Nature Science Foundation of China(Grant No.11665011);Gansu Provincial Science and Technology Program(Grant No.18JR2JG001).

摘　　要：Carbon-ion irradiation is a technique for trait improvement in the microalgae,but the underlying mechanisms that how it altered the biomass,and photosynthetic pigments accumulation were unclear.One mutant(DS240G-1)was obtained from Dunaliella salina by heavy ion irradiation mutagenesis.Compared to the wild type,the biomass accumulation and maximum growth rate of DS240G-1 were increased by 34%and 55%respectively,and itsβ-carotene content was 21%higher than the wild type.Subsequent analysis of the chlorophyll fluorescence parameters indicated that higherβ-carotene productivity was likely owing to the improved maximum quantum efficiency(Fv/Fm)and decreased thermal dissipation of photosynthesis in DS240G-1 than that of wild type during cultivation.In addition,the result of this study revealed that high content of ROS may induceβ-carotene accumulation in mutant DS240G-1.Also,the total fatty acid(TFA)content in mutant DS240G-1 was 79%higher than that in wild type.Owing to its highβ-carotene productivity and total fatty acid content,DS240G-1 could be considered as a promising candidate for microalgaeβ-carotene and biodiesel production.This work provided the first insight into the biological effects involved in carbon-ions irradiation on the photosynthetic activity of D.salina.

关 键 词：MICROALGAE D.salina carbon-ions irradiation chlorophyll fluorescence Β-CAROTENE 

分 类 号：Q94[生物学—植物学]