涉及簇毛麦6V染色体结构变异体的筛选与鉴定  

Screening and Identification of Chromosomal Aberrations Involving Haynaldia villosa Chromosome 6V

作　　者：张伟 ZHANG Wei(Tongling Agricultural Technology Management Service Center,Tongling 244000,China)

机构地区：[1]铜陵市农业技术管理服务中心,安徽铜陵244000

出　　处：《安徽农学通报》2021年第17期21-25,33,共6页Anhui Agricultural Science Bulletin

摘　　要：簇毛麦是小麦的一个野生近缘种,抗病性、抗逆性强,蛋白质和赖氨酸含量高,是小麦遗传改良的重要基因源。为定位、转移和利用簇毛麦有益基因,南京农业大学细胞遗传所通过花粉辐射,获得了一批涉及簇毛麦不同染色体且不同区段的结构变异体。该研究从这些结构变异体中筛选鉴定涉及簇毛麦6V染色体的结构变异体,共筛选鉴定出13种涉及簇毛麦6V染色体的结构变异体,包括2个整臂易位、5个小片段易位、4个大片段易位、1个缺失和1个中间插入易位,这些结构变异体的筛选鉴定将为进一步定位和利用簇毛麦6V染色体上的优异基因奠定基础。Haynaldia villosa(syn. Dasypyrum villosum)is a wild relative species of wheat(Triticum aestivum). It has strong ability of diseases and stress resistance, high protein and lysine contents. These characters make it an important gene pool for wheat genetic improvement. To localize, transfer and use interested genes of H. villosa, a series of involving different H. villosa chromosome and chromosomal region lines induced by irradiation have been developed at the Cytogenetics Institute, Nanjing Agricultural University, China. The aim of this study is to screen and identify structural changes involving H. villosa chromosome 6V in these chromosomal aberrations. Finally, 13 types of chromosomal aberrations involving H. villosa chromosome 6V were identified, including 2 of whole arm translocations, 5 of small alien segment translocation, 4 of large alien segment translocation, 1 of chromosomal deletion and 1of intercalary translocation. Screening and identification of these chromosomal aberrations will be basis to localize and use interested genes of H. villosa chromosome 6V.

关 键 词：小麦 簇毛麦 染色体结构变异体 基因组原位杂交 6V特异分子标记 

分 类 号：S512.1[农业科学—作物学]