Application and optimization of solid-state fermentation process for enhancing polygalacturonase production by Penicillium expansum  

作　　者：Mingming Zhu Hongju He Mingtao Fan Hanjun Ma Haiwei Ren Jie Zeng Haiyan Gao 

机构地区：[1]School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,China [2]College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China [3]School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China

出　　处：《International Journal of Agricultural and Biological Engineering》2018年第6期187-194,共8页国际农业与生物工程学报（英文）

基　　金：The authors would like to thank the High-Level Talent Scientific Research Staring Foundation(No.2015015)provided by Henan Institute of Science&Technology;Key Scientific Research Project(17A550001)supported by Education Department of Henan Province;Program for Innovative Research Talents(in Science and Technology)of Henan Provincial University(16HASTIT015);Excellent Youth Foundation of Henan Scientific Committee(174100510003).

摘　　要：Penicillium expansum 3.5425 was applied in solid-state fermentation(SSF)of agricultural wastes for polygalacturonase biosynthesis.Among various carbon additives,apple pomace was most suitable for the biosynthesis of polygalacturonase(1440.57 U/g).Optimization of medium parameters using rotational orthogonal design(ROD)experiment combined with optimal fermentation conditions resulted in a 2.72-fold increase in the polygalacturonase production.By using ammonium sulphate precipitation,ion-exchange and gel-permeation chromatography,the polygalacturonase produced by P.expansum 3.5425 was finally purified which had specific activity of 19269 U/mg and molecular weight of 30 kDa.The enzyme was remarkably active in the pH range of 3-5 and at 50℃,which makes it more acceptable in the industrial application.Besides,partially purified polygalacturonase(875.15 U/mL)was used for apple juice clarification and the clarity at 0.4 mL/kg was maximum,which reveals a great potential of polygalacturonase in food industry.

关 键 词：solid-state fermentation(SSF) POLYGALACTURONASE Penicillium expansum juice clarification agricultural wastes 

分 类 号：TQ9[化学工程]