挥发性化合物2-甲基苯并噻唑对核盘菌和灰葡萄孢的抑制效应  

The inhibitory effect of the volatile compound 2-methyl benzothiazole on pathogenic fungi Sclerotinia sclerotiorum and Botrytis cinerea

作　　者：扈景晗 王东 东保柱 孟焕文[1] 赵君[1,2] 周洪友 Hu Jinghan;Wang Dong;Dong Baozhu;Meng Huanwen;Zhao Jun;Zhou Hongyou(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010020,Inner Mongolia Autonomous Region,China;Inner Mongolia Cold and Arid Region Crop Protection Engineering Technology Center,Hohhot 010020,Inner Mongolia Autonomous Region,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,呼和浩特010020 [2]内蒙古寒旱区作物保护工程技术中心,呼和浩特010020

出　　处：《植物保护学报》2021年第4期781-788,共8页Journal of Plant Protection

基　　金：国家自然科学基金(31260445,31960533);国家重点研发计划(2017YFD0201101)。

摘　　要：为明确挥发性化合物2-甲基苯并噻唑(2-methylbenzothiazole,2-MBTH)对向日葵菌核病病原菌核盘菌Sclerotinia sclerotiorum和番茄灰霉病病原菌灰葡萄孢Botrytis cinerea的防治潜力,采用密封盘菌丝生长速率法测定其对2种病原菌的抑制作用,利用离体叶片接种试验分析其对2种病原菌致病力的影响。结果显示,2-MBTH对核盘菌和灰葡萄孢具有明显的抑制作用,2-MBTH对核盘菌菌丝的EC_(50)为0.87μL/L,对灰葡萄孢菌丝的EC_(50)为4.49μL/L。2-MBTH能够显著降低灰葡萄孢的致病力,受抑制程度与2-MBTH浓度呈正相关;扫描电镜观察发现,2-MBTH能够使灰葡萄孢菌丝形态异常,菌丝干瘪,分枝增多,表面塌陷;使核盘菌菌丝破损,表面塌陷。另外,随着2-MBTH熏蒸浓度的增大,核盘菌的菌核数量和质量都有所减少,且健康菌核在2-MBTH的熏蒸作用下失去了萌发能力。表明2-MBTH对核盘菌和灰葡萄孢均有较高的抑制作用,表现为菌丝生长、菌核萌发受到抑制,利用其熏蒸作用可以为向日葵菌核病和番茄灰霉病的有效防控提供新思路。In order to evaluate the biocontrol potentiality of the volatile compound 2-methylbenzothiazole(2-MBTH) to pathogenic fungi Sclerotinia sclerotiorum and Botrytis cinerea, its effects on mycelium growth rate were determined on PDA plates and its influence on the pathogenicity of the two pathogens was analyzed via in vitro leaf inoculation. The results showed that 2-MBTH had obvious inhibitory effects on the mycelium growth of S. sclerotiorum and B. cinerea. The EC_(50) of 2-MBTH on the mycelium growth of S. sclerotiorum and B. cinerea were 0.87 μL/L and 4.49 μL/L, respectively. Fumigation with 2-MBTH could significantly reduce the pathogenicity of B. cinerea, and the degree of inhibition was positively correlated with the concentration of 2-MBTH. Results of scanning electron microscope observation showed that 2-MBTH could lead to pits on the hyphal surface, and increased hyphal branches of S. sclerotiorum and B. cinerea. In addition, it was found that the increase of fumigation concentration with 2-MBTH could reduce the number and weight of sclerotia formed by S. sclerotiorum on PDA plates, and normal healthy sclerotia could lose their ability to germinate after treatment with 2-MBTH.These results indicated that 2-MBTH was highly inhibitory to both S. sclerotiorum and B. cinerea, and exhibited a promising potential to be used as a fumigant for controlling sunflower sclerotium blight and tomato grey mould.

关 键 词：2-甲基苯并噻唑 向日葵菌核病 番茄灰霉病 抑制效应 

分 类 号：S432.4[农业科学—植物病理学]