香茅精油微乳液对朱砂叶螨的生物活性及其解毒酶系活性的影响  

Bioactivities of citronella oil microemulsion against Tetranychus cinnabarinus (Acari:Tetranychidae) and its effect on the detoxification enzyme activities

作　　者：程作慧[1] 樊芳芳 王旗[1] 刘庆[1] 张鹏[1] 李锐[2] 李生才[2] Cheng Zuohui;Fan Fangfang;Wang Qi;Liu Qing;Zhang Peng;Li Rui;Li Shengcai(College of Basic Department,Shanxi Agricultural University,Taigu 030801,Shanxi Province,China;College of Agriculture,Shanxi Agricultural University,Taigu 030801,Shanxi Province,China)

机构地区：[1]山西农业大学基础部,太谷030801 [2]山西农业大学农学院,太谷030801

出　　处：《植物保护学报》2021年第4期914-920,共7页Journal of Plant Protection

基　　金：山西省自然科学基金(201801D121203);山西农业大学科技创新基金(2016ZZ05)。

摘　　要：为明确香茅精油微乳液对朱砂叶螨的生物活性及其解毒酶系活性的影响,采用熏蒸法测定香茅精油微乳液对朱砂叶螨的熏蒸活性及香茅精油微乳液亚致死剂量处理下朱砂叶螨的产卵情况、趋避情况及解毒酶系活性。结果表明,当剂量达到2.5μL时,香茅精油微乳液处理下朱砂叶螨的死亡率达到80.37%以上;香茅精油微乳液熏蒸24 h时,其对朱砂叶螨的亚致死剂量LD_(10)和LD_(30)分别为0.082μL和0.284μL;香茅精油微乳液LD_(10)和LD_(30)处理24 h时,其对朱砂叶螨的产卵抑制率分别为76.30%和78.67%,对朱砂叶螨的趋避率分别为80.00%和88.00%;香茅精油微乳液LD_(10)和LD_(30)处理后,朱砂叶螨体内酯酶活性在0~8 h表现出激活作用,随后一直处于抑制状态,谷胱甘肽-S-转移酶活性一直处于激活状态,而多功能氧化酶活性处于抑制状态。表明香茅精油微乳液是一种很有前景的绿色杀螨剂。To clarify the bioactivities of citronella oil microemulsion against carmine spider mite Tetranychus cinnabarinus and its effect on the activity of detoxification enzymes,the fumigation activity of citronella oil microemulsion and the oviposition inhibitory activity,repellent activity and detoxification enzyme activity at the sublethal dosages of citronella oil microemulsion against T.cinnabarinus were determined using the fumigation method.The results showed that the mortality of T.cinnabarinus caused by citronella oil microemulsion reached more than 80.37%at the dosage of 2.5μL.The sublethal dosages of LD_(10) and LD_(30) were 0.082μL and 0.284μL of citronella oil microemulsion by fumigating T.cinnabarinus for 24 h.By fumigating with citronella oil microemulsion at LD_(10) and LD_(30) for 24 h,the oviposition inhibition rates were 76.30%and 78.67%,and the repellency rates were 80.00%and 88.00%,respectively.The esterase activity was up-regulated in eight hours but then down-regulated;the glutathione-S-transferase activity was up-regulated but the mixed function oxidase activity was downregulated when treated with citronella oil microemulsion at LD_(10) and LD_(30).It indicated that citronella oil microemulsion was a promising green acaricide.

关 键 词：香茅精油 微乳液 朱砂叶螨 熏蒸活性 酶 

分 类 号：S433.7[农业科学—农业昆虫与害虫防治]