噬菌体重组酶系统在合成生物学中的应用  

Application of Phage Recombinase Systems in Synthetic Biology

作　　者：郭曼曼 田开仁 乔建军[1,2,3] 李艳妮 GUO Man-man;TIAN Kai-ren;QIAO Jian-jun;LI Yan-ni(Department of Pharmaceutical Engineering,College of Chemical Engineering,Tianjin University,Tianjin 300072,China;Key Laboratory of Systematic Bioengineering,Ministry of Education,Tianjin University,Tianjin 300072,China;Synthetic Biology Platform,Tianjin Collaborative Innovation Center of Chemistry and Chemical Engineering,Tianjin 300072,China)

机构地区：[1]天津大学化工学院制药工程系,天津300072 [2]天津大学系统生物工程教育部重点实验室,天津300072 [3]天津化学化工协同创新中心合成生物学平台,天津300072

出　　处：《中国生物工程杂志》2021年第8期90-102,共13页China Biotechnology

基　　金：国家自然科学基金(31770076)资助项目。

摘　　要：合成生物学技术采用工程化设计理念,对生物体进行有目标的设计、改造乃至重新合成,对重塑非自然功能的"人造生命"具有重要意义。噬菌体重组系统具有高效、精确和广谱适用性等特点,在基因工程、代谢工程以及生物治疗等合成生物学领域得到了广泛的应用。从基因电路、体内遗传改造和体外重组等方面全面阐述了噬菌体重组系统在合成生物学研究的现状及热点,对当前该系统的局限性进行了探讨,并就未来的研究和发展趋势进行了展望。Synthetic biology is an emerging filed that aims to de novo design and synthesis of biological functional modules,gene circuits or artificial life with the standardized bioparts based on engineering principles.Since its inception in 1998,phage recombinase-mediated site-specific recombination,also known as recombineering,has revolutionized synthetic biology.Due to high efficiency,high precision,and broad-spectrum applicability,phage recombinases have been developed into powerful synthetic biology tools such as gene editing,DNA assembly,and gene-directed insertion.Yet,some applications,such as gene circuits,biocomputing,and biotherapy,need controlled excision,and this requires the number of well-characterized integrases and RDFs.Here,the classification and applicability of phage recombinases and focused on the application of phage recombinase systems in gene circuits design and construction,in vivo genetic modification and in vitro recombination were briefly introduced.Besides,the challenges of phage recombinase as tools for genetic engineering and sophisticated gene expression regulation as well as prospects for phage recombinases were analyzed.

关 键 词：合成生物学 噬菌体重组酶 基因电路 遗传改造 体外重组 

分 类 号：Q814[生物学—生物工程]