高频电刺激对大鼠海马脑片癫痫样放电特性影响的研究  

Effect of High Frequency Electrical Stimulation on Epileptiform Discharge Characteristics in Rat Hippocampal Slices

作　　者：张康辉 李成双 东磊 王慧泉[1] 郑羽[1] ZHANG Kang-Hui;LI Cheng-Shuang;DONG Lei;WANG Hui-Quan;ZHENG Yu(School of Life Sciences,Tiangong University,Tianjin 300387,China;State Key Laboratory of Precision Measurement Technology and Instruments,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津工业大学生命科学学院,天津300387 [2]天津大学精密测量技术与仪器国家重点实验室,天津300072

出　　处：《生物化学与生物物理进展》2021年第8期947-958,共12页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(62071329,61871288);天津市自然科学基金(20JCYBJC00130);载人航天工程航天医学实验领域项目(HYZHXM03003)资助。

摘　　要：本文采用电极阵列检测技术,在大鼠海马脑切片上诱导出稳定的癫痫样放电,分析、研究130 Hz的高频电刺激(high-frequency stimulation,HFS) CA3区时,海马切片在癫痫发作间期放电(inter-ictal discharges,IID)和发作期放电(ictal discharges,ID)的各项参数、癫痫样放电地起始位点、传播方向和传输速率以及各频段的功率谱密度.结果显示:高频电刺激可以有效地降低癫痫发作期的幅值、减少持续时间、增长潜伏时间、抑制癫痫样放电由IID向ID的转变等.提示高频电刺激抑制癫痫的作用机制是通过促进神经元之间的抑制性传输系统,并且抑制海马神经元之间的兴奋性连接,从而达到抑制效果.In this paper, stable epileptiform discharges were induced on rat hippocampal slices by using the electrode array detection technique. To analyze and study the parameters of the hippocampal slices, the starting site of epileptiform discharges, the propagation direction and transmission rate, and the power spectral density of each frequency band during the inter-ictal discharges(IID) and ictal discharges(ID) when 130 Hz high-frequency stimulation(HFS) was applied to CA3 region. The results showed that HFS could effectively reduce the amplitude and duration of seizures, increase latency time, and inhibit the transition of epileptiform discharges from IID to ID. In conclusion, HFS resists epilepsy through promoting the inhibitory transmission system and resisting the excitatory connections between hippocampal neurons.

关 键 词：高频电刺激 癫痫样放电 海马切片 电极阵列 

分 类 号：R338.8[医药卫生—人体生理学] R742.1[医药卫生—基础医学]