Biolog微平板分析在微生物实验教学中的应用  

Application of Biolog microplate analysis in microbiology experiment

作　　者：姚菁华[1] 叶景法 肖雷[1] 谭添 YAO Jinghua;YE Jingfa;XIAO Lei;TAN Tian(School of Chemical Engineering and Technology,China University of Mining&Technology,Xuzhou 221116,China)

机构地区：[1]中国矿业大学化工学院,江苏徐州221116

出　　处：《实验室科学》2021年第3期125-126,131,共3页Laboratory Science

基　　金：国家自然科学基金青年基金(项目编号:51404259);中国矿业大学教学改革项目(项目编号:2020YB27)。

摘　　要：微生物群落多样性分析有助于了解群落功能微生物的结构、生长生态特征、代谢活性和功能等,是许多学校微生物实验教学必开的实验项目之一。Biolog微平板分析法是利用氧化还原反应原理,定量描述土壤微生物对不同碳源的利用能力,该方法是目前研究微生物群落多样性最简单和快速的方法,将其用于微生物教学实践中具有很好的应用与推广前景。在分析Biolog微平板原理的基础上,阐述了Biolog微平板的优点及其在相关微生物教学实验中的应用。Microbial community diversity analysis is helpful to understand the structure,growth and ecological characteristics,metabolic activity and function of community functional microorganisms.It is one of the necessary experimental items for microbial experiment teaching in many universities.Biolog microplate analysis is based on the redox reaction as the basic reaction condition,which can quantitatively describe the ability of soil microorganisms to use different carbon sources.This method is currently the simplest and fastest method to study the diversity of microbial communities.It has good application and promotion prospects in experiment teaching.Based on the analysis of the principle of Biolog microplate,the advantages and applications in related microbiology teaching experiments were discussed.

关 键 词：Biolog微平板 微生物教学 微生物群落 

分 类 号：G642[文化科学—高等教育学]