利用微卫星分子标记分析渤海湾的口虾蛄遗传多样性  

Genetic Diversity of Wild Populations of Mantis Shrimp Oratosqilla oratoria in Bohai Bay Using Microsatellite Markers

作　　者：谷德贤[1] 王婷 许玉甫 吴宁[1] 王娜[1] 郝郁[1] 王刚[1] 李文雯[1] 刘国山[1] GU Dexian;WANG Ting;XU Yufu;WU Ning;WANG Na;HAO Yu;WANG Gang;LI Wenwen;LIU Guoshan(Tianjin Fisheries Research Institute,Tianjin 300221,China;Hebei Ocean and Fisheries Science Research Institute,Qinhuangdao 066200,China)

机构地区：[1]天津市水产研究所,天津300221 [2]河北省海洋与水产科学研究院,河北秦皇岛066200

出　　处：《水产科学》2021年第5期693-699,共7页Fisheries Science

基　　金：天津市水产局青年科技创新项目(J2016-04青);天津市水产研究所科研项目.

摘　　要：2019年11月在渤海湾的秦皇岛、唐山、昌黎、天津和黄骅海域,用单拖网采集口虾蛄活样本,取背部肌肉,采用12对微卫星引物,分析渤海湾5个海域口虾蛄自然群体的遗传多样性。研究结果表明,5个群体的等位基因数为3~16,平均有效等位基因数为2.9063~3.4102,平均观测杂合度为0.5004~0.5342,平均期望杂合度为0.5310~0.5491,平均多态信息含量为0.5422~0.6038;哈代温伯格平衡检验表明,5个群体81.67%位点处于平衡状态。天津群体与唐山群体的遗传距离最远,相似度最低。系统进化树结果显示,秦皇岛群体与唐山群体聚在一起,昌黎群体与黄骅群体聚在一起,而后又与天津群体聚在一起。综上所述,5个群体遗传多样性水平较高,具有一定的育种潜力,可以作为良好的育种材料。The genetic diversity was analyzed in the back muscles of wild populations of mantis shrimp Oratosqilla oratoria sampled in the sea areas of Qinhuangdao,Tangshan,Changli,Tianjin and Huanghua by a single layer gill net in November 2019 by 12 pairs of microsatellite primers.The results showed that the five populations had from 3 to 16 in the allele number with the average effective alleles ranging from 2.9063 to 3.4102,the average observed heterozgosity(H_(o))ranging from 0.5004 to 0.5342,average expected heterozgosity(H_(e))from 0.5310 to 0.5491,and average polymorphic information content(PIC)ranging from 0.5422 to 0.6038.Hardy-Weinberger equilibrium test revealed that 81.67%position was in balance in the five populations.There was the maximal genetic distance between Tianjin and Tangshan populations,and the minimal genetic identity between Tianjin and Tangshan populations.The UPGMA phylogentic tree showed that the populations of Qinhuangdao and Tangshan were clustered together,Changli and Huanghua were clustered together,and then they got together with the Tianjin population.The findings indicated that the five mantis shrimp populations had high levels of genetic diversity,being the potential as breeding material and used as breeding material.

关 键 词：渤海湾 口虾蛄 微卫星 遗传多样性 

分 类 号：S917[农业科学—水产科学]