基于凋亡机制院内制剂抗瘤丸优化方-4对U87裸鼠移植瘤动物模型实验研究  

Based on the mechanism of apoptosis KLW-4 on the optimized party U87 nude mice transplantation tumor animal model of experimental research

作　　者：冯英楠 陈菲[1] 王晓萌[1] 王海征[1] 林晓兰[1] FENG Yingnan;CHEN Fei;WANG Xiaomeng;WANG Haizheng;LIN Xiaolan(Xuanwu Hospital of Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学宣武医院药学部,北京100053

出　　处：《环球中医药》2021年第9期1561-1565,共5页Global Traditional Chinese Medicine

基　　金：北京市科委“十病十药”专项(Z171100001717006)。

摘　　要：目的探讨中药复方制剂抗瘤丸优化方-4治疗神经胶质瘤的作用机制。方法制备人神经胶质瘤U87细胞BALB/c裸鼠移植瘤模型,按瘤块体积和体质量分层随机法分为:模型对照组、阳性药(替莫唑胺)对照组、抗瘤丸优化方-4高、中、低剂量组,每组12只,连续给药20天。检测各组裸鼠瘤块微血管密度(microvascular density,MVD)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、凋亡细胞率。结果与模型组比较,抗瘤丸优化方-4低剂量组,阳性药对照组的MVD值明显降低(P<0.05),CD34低表达;抗瘤丸优化方-4高、中、低剂量组,阳性药对照组移植瘤灰度值明显增高(P<0.05),VEGF低表达;抗瘤丸优化方-4高、中、低剂量组,阳性药对照组可见大量的凋亡细胞。与模型对照组比较,上述给药组细胞凋亡率显著增高(P<0.05)。结论抗瘤丸优化方-4治疗胶质瘤的作用机制可能与降低MVD,抑制VEGF表达,促进细胞凋亡相关。Objective To demonstrate the mechanism of the treatment of glioma by compound Chinese medicine Kangliuwan(KLW)-4.Methods BALB/c nude mice transplantation tumor animal model was induced by U87 glioma cells,and randomly divided into five groups:the model control group,the temozolomide(TMZ)group(positive drug control group),the KLW-4 high,medium and low dose groups,with 12 mice in each group.After 20 days of continuous drug administration.The MVD,VEGF and cell apoptosis rate were observed to demonstrate the mechanism of KLW-4.Results Compared with the model control group,the low dose KLW-4,TMZ group of tumor MVD decreased(P<0.05),TMZ group of tumor CD34 value decreased.Low,middle,high dose KLW-4 group(P<0.05),TMZ group of tumor VEGF value decreased;the low,middle,high dose KLW-4 group and the TMZ group of the apoptosis rate increased(P<0.05).Conclusion KLW-4 has good therapeutic effect on U87 BALB/c nude mice transplantation tumor animal model.The protective effects about KLW-4 may be associated with decreased the expression of MVD,inhibited the expression of VEGF and promoted apoptosis.

关 键 词：抗瘤丸 神经胶质瘤 优化处方 作用机制 凋亡 移植瘤模型 

分 类 号：R285.5[医药卫生—中药学]