高效液相色谱法测定隐形眼镜护理液中聚乙烯吡咯烷酮  

Determination of polyvinylpyrrolidone in contact lens care solution by high performance liquid chromatography

作　　者：徐萍华 张莉 韩银 鲍娇慧 诸丹 徐畅 Xu Pinghua;Zhang Li;Han Yin;Bao Jiaohui;Zhu Dan;Xu Chang(Zhejiang Institute of Medical Device Supervision and Testing,Hangzhou 310018,China)

机构地区：[1]浙江省医疗器械检验研究院,杭州310018

出　　处：《化学分析计量》2021年第9期34-37,共4页Chemical Analysis And Meterage

基　　金：浙江省省属科研院所扶持经费项目(20190002)。

摘　　要：建立高效液相色谱法测定隐形眼镜护理液中聚乙烯吡咯烷酮含量的方法,并经方法学验证其可行性。以20 mmol/L氯化钠作为流动相,采用Oligo-SAX强阴离子交换色谱柱分离,检测波长为220 nm。聚乙烯吡咯烷酮的质量浓度在0.01~0.50 mg/mL范围内与色谱峰面积呈良好的线性关系,线性相关系数为0.9990,检出限为0.0003 mg/mL,定量限为0.001 mg/mL。测定结果的相对标准偏差为1.2%(n=6),日间重复性为1.7%(n=6),样品加标回收率为90.1%~104%。该方法可作为角膜接触镜护理液中有效成分聚乙烯吡咯烷酮质量控制的方法之一。A method for the determination of polyvinylpyrrolidone in contact lens care solution by high performance liquid chromatography was established,and its feasibility was verified by methodology.20 mmol/L sodium chloride was used as mobile phase and separated by Oligo-SAX of strong anion exchange column.The detection wavelength was 220 nm.The mass concentration of polyvinylpyrrolidone had a good linear relationship with the chromatographic peak area in the range of 0.01-0.50 mg/mL.the linear correlation coefficient was 0.9990,the detection limit was 0.0003 mg/mL and the quantitative limit was 0.001 mg/mL.The relative standard deviation of the determination results was 1.2%(n=6),the daytime repeatability was 1.7%(n=6),and the recoveries were 90.1%-104%.The method can be used as one of the methods for the quality control of polyvinylpyrrolidone,which is an effective component in corneal contact lens nursing solution.

关 键 词：隐形眼镜护理液 聚乙烯吡咯烷酮 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]