雷公藤内酯酮脂质体的体外释放特性及体外抗肿瘤活性  

In vitro Release Characteristics and in vitro Antitumor Activity of Triptonide Liposomes

作　　者：戴雅彬 黄慧[1] 张月芬[1] 郭丹[1] DAI Ya-bin;HUANG Hui;ZHANG Yue-fen;GUO Dan(Science and Technology Service Center,Fujian Health College,Fuzhou 350101,China)

机构地区：[1]福建卫生职业技术学院科技服务中心,福建福州350101

出　　处：《海峡药学》2021年第8期14-17,共4页Strait Pharmaceutical Journal

基　　金：福建省中青年教师教育科研项目(JZ180547);福建卫生职业技术学院校级科研项目(2019-1-07)。

摘　　要：目的考察雷公藤内酯酮(Triptonide,TPN)脂质体的体外释放特性及体外抗肿瘤活性。方法反渗透法考察脂质体的体外释放行为;MTT法测定其在体外对人结肠癌细胞SW620和人白血病细胞K562的抗肿瘤活性。结果TPN脂质体在pH 6.8的缓冲环境中的释放符合一级动力学方程,对SW620和K562细胞48 h的半数抑制浓度显著低于TPN溶液。结论TPN脂质体在体外具有缓释特性,能增强TPN的体外抗肿瘤活性,有望开发为临床药用新剂型。OBJECTIVE To investigate the in vitro release characteristics and anti-tumor activity of triptonide(Triptonide,TPN)liposomes.METHODS Thereverse dialysis method was used to investigate the in vitro release behavior of TPN liposomes.The MTT method was used to detect its anti-tumor activity of human colon cancer cells SW620 and human leukemia cells K562 in vitro.RESULTS The release of TPN liposomes conformed to the first-order kinetic equation in a buffer environment of pH 6.8.The half inhibitory concentration of TPN liposomes in 48 h for SW620 and K562 cells was significantly lower than that of TPN solution.CONCLUSION TPN liposomes have sustained release properties in vitro,the antitumor activity of TPN could be enhanced by TPN liposomes in vitro.The TPN liposomes expected to be developed as a new dosage form for clinical medicine.

关 键 词：雷公藤内酯酮 脂质体 体外释放 抗肿瘤 

分 类 号：R965[医药卫生—药理学]