慢性肾小球肾炎患者血清lncRNA XIST、miR-217表达及其预后相关性研究  

Study on the expressions of IncRNA XIST and miR-217 in serum of patients with chronic glomerulonephritis and their prognostic correlation

作　　者：范佳妮 牟利军[3] 陶招娣[1,4] 王颖 FAN Jia-ni;MOU Li-jun;TAO Zhao-di;WANG Ying(Department of Nephrology,Taizhou Hospital,Taizhou,Zhejiang 317000,China;不详)

机构地区：[1]浙江省台州医院肾内科,浙江台州317000 [2]台州恩泽医疗中心(集团)恩泽医院肾内科,浙江台州318050 [3]浙江大学医学院附属第二医院肾内科,浙江杭州310009 [4]台州恩泽医疗中心(集团)恩泽医院血透室,浙江台州318050

出　　处：《中国卫生检验杂志》2021年第16期1971-1974,1979,共5页Chinese Journal of Health Laboratory Technology

基　　金：浙江省医药卫生科技计划项目(2017KY203)。

摘　　要：目的检测慢性肾小球肾炎患者血清长链非编码RNA X染色体失活特异转录物(Lnc RNA XIST)和微小RNA-217(mi R-217)的表达情况,并探究其表达水平与慢性肾小球肾炎患者预后的相关性。方法选取2015年12月—2017年4月浙江省台州医院收治的慢性肾小球肾炎患者70例作为研究组,同期选取在医院进行常规体检的健康者70例作为对照组。采用实时荧光定量PCR(q RT-PCR)法检测所有研究对象血清lnc RNA XIST、mi R-217表达水平;通过研究对象工作特征曲线(ROC)评估血清lnc RNA XIST、mi R-217表达水平对慢性肾小球肾炎的诊断价值;分析患者预后不良情况发生率;并采用Cox回归分析患者发生预后不良的影响因素。结果研究组血清lnc RNA XIST和mi R-217表达水平均显著高于对照组,差异有统计学意义(P <0.05)。ROC结果显示,血清lnc RNA XIST和mi R-217诊断慢性肾小球肾炎的AUC分别为0.903、0.797,截断值分别为0.843、1.197,此时对应灵敏度分别为88.6%、70.0%,特异度分别为75.7%、78.6%。血清lnc RNA XIST和mi R-217联合检测慢性肾小球肾炎的AUC为0.941,此时对应灵敏度和特异度分别为94.3%、74.3%。Cox回归分析结果显示,血清lnc RNA XIST高表达和mi R-217高表达是影响慢性肾小球肾炎患者预后的独立危险因素。结论血清lnc RNA XIST和mi R-217可作为慢性肾小球肾炎的早期诊断及预后评估的潜在生物学指标。Objective To detect the expressions of long non-coding RNA X-chromosome inactivation specific transcript( Lnc RNA XIST) and micro RNA-217( mi R-217) in serum of patients with chronic glomerulonephritis,and to explore the correlation between the levels and the prognosis of patients with chronic glomerulonephritis. Methods A total of 70 patients with chronic glomerulonephritis admitted to Taizhou Hospital from December 2015 to April 2017 were selected as the study group,and 70 healthy people who had routine examination in our hospital were selected as the control group at the same time.The levels of serum Lnc RNA XIST and mi R-217 were detected by real-time fluorescent quantitative PCR( q RT-PCR);the diagnostic value of serum lnc RNA XIST and mir-217 levels in patients with chronic glomerulonephritis was evaluated by receiver operating characteristic curve( ROC);the incidence of poor prognosis was analyzed;and Cox regression was used to analyze the influencing factors of poor prognosis. Results The levels of Lnc RNA XIST and mi R-217 in the study group were significantly higher than those in the control group,with the difference statistical significance( P < 0. 05). ROC results showed that AUC of serum Lnc RNA XIST and mi R-217 in the diagnosis of chronic glomerulonephritis was 0. 903 and 0. 797 respectively,and the cutoff value was 0. 843 and 1. 197 respectively;at this time,the sensitivity was 88. 6% and 70. 0%,and the specificity was 75. 7% and 78. 6%,respectively. The AUC of serum Lnc RNA XIST combined with mi R-217 in chronic glomerulonephritis was 0. 941,and the sensitivity and specificity were 94. 3% and 74. 3%,respectively. Cox regression analysis showed that the high expression of serum lnc RNA XIST and mir-217 are independent risk factors for the prognosis of patients with chronic glomerulonephritis. Conclusion Serum Lnc RNA XIST and mi R-217 can be used as potential biological indicators for early diagnosis and prognosis evaluation of chronic glomerulonephritis.

关 键 词：慢性肾小球肾炎 长链非编码RNA X染色体失活特异转录物 微小RNA-217 预后 相关性 

分 类 号：R692.3[医药卫生—泌尿科学]