天山北麓典型水库病毒多样性及其中潜在病原体的分析  

Potential Etiological Agent and Diversity of Viral Community in Reservoirs in Northern Part of Tianshan Mountains

作　　者：张政 常娜娜 杜菲 地丽胡玛·阿吉 张成 马正海[1] 毕玉海 ZHANG Zheng;CHANG Nana;DU Fei;DILIHUMA Aji;ZHANG Cheng;MA Zhenghai;BI Yuhai(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China;CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046 [2]中国科学院微生物研究所,中国科学院病原微生物与免疫学重点实验室,北京100101

出　　处：《环境科学研究》2021年第9期2264-2272,共9页Research of Environmental Sciences

基　　金：新疆维吾尔自治区区域协同创新专项(上海合作组织科技伙伴计划)项目(No.2017E01022);国家自然科学基金项目(No.41761096)。

摘　　要：为了解天山北麓中段水库中病毒多样性及其功能和潜在危害,选取三屯碑水库(STW)、八一水库(BYW)和蘑菇湖水库(MGW)为典型水库进行病毒宏基因组学分析.水库水样经FeCl 3絮凝和聚乙二醇(polyethylene glycol,PEG)富集获得浓缩病毒颗粒,利用病毒宏基因组学方法分析病毒多样性及其功能,挖掘并分析病原体的基因序列.结果表明:①天山北麓中段典型水库STW、BYW和MGW中分别获得病毒序列36784178、32434254和30537928条,形成重叠群15400、11894和30771个.②水库病毒分属9科、26种,在科分类阶元上优势病毒均属于有尾噬菌体目(Caudovirales),BYW和MGW中肌尾噬菌体科(Myoviridae)的相对丰度最高,STW中长尾噬菌体科(Siphoviridae)的相对丰度最高;在种分类阶元上,STW、BYW和MGW中相对丰度最高的病毒分别为链霉菌噬菌体(Streptomyces phages)、微囊藻噬菌体(Microcystis phages)和欧文氏菌噬菌体(Erwinia phages).③病毒功能基因分析表明,噬菌体主要衣壳蛋白和尾蛋白等结构蛋白以及病毒复制和装配相关蛋白编码基因的相对丰度较高.④水库中存在多种植物和动物的致病性病毒,其中MGW中肠病毒相对丰度较高,并从中获得了人类手足口病的病原体——柯萨奇病毒A10(Coxsackievirus A10,CV-A10)的VP1基因序列,其与原始株Kowalik序列一致.研究显示:天山北麓中段水库优势病毒均为噬菌体,病毒复制活跃;不同水库的病毒群落组成存在差异,其与周边环境和人类活动等因素相关;水库中检测出CV-A10等致病性病毒,存在潜在公共卫生风险.In order to investigate the diversity,function and potential harm of viral community in the reservoirs at the middle part of the Northern Tianshan Mountains,Santunbei Reservoir(STW),Bayi Reservoir(BYW)and Moguhu Reservoir(MGW)were selected as typical reservoirs.Concentrated virus particles were obtained from the reservoir water samples by FeCl 3 flocculation and enriched by polyethylene glycol(PEG).The diversity and function of the viruses were then analyzed by viral metagenomics,and the genetic sequences of pathogens were analyzed.The results showed that:(1)From the typical reservoirs of STW,BYW and MGW at the middle part of Northern Tianshan Mountain,36,784,178,32,434,254 and 30,537,928 viral sequences were obtained,and 15,400,11,894 and 30,771 contigs were formed,respectively.(2)The viruses from the reservoirs belonged to 9 families and 26 species,among which 3 families belonging to Caudovirales were found to be the dominant viruses at the virus family level.Myoviridae showed a highly relative abundance in BYW and MGW,while Siphoviridae showed relative high abundance in STW.At the species level,Streptomyces phages,Microcystis phages and Erwinia phages exhibited relative high abundance in STW,BYS and MGW,respectively.(3)Viral gene analysis indicated that the genes related to capsid protein and tail protein as well as viral replication and assembly showed relatively a high abundance.(4)Several pathogens of plants and animals were found in the reservoirs,among which enterovirus showed relatively abundant in MGW,and the VP1 gene sequence of Coxsackievirus A10(CV-A10)obtained was consistent with the original Kowalik strain.In summary,bacteriophages were all the dominant viruses in the reservoirs,and the virus replicated actively.The composition of the viral communities in different reservoirs was different,which could be related to the surrounding environment and human activities.The results of this study indicate that the presence of pathogenic viruses(CV-A10,for instance)in the reservoirs could be a potential ris

关 键 词：天山北麓 水库 病毒宏基因组学 病毒多样性 柯萨奇病毒A10 

分 类 号：X52[环境科学与工程—环境工程] R123[医药卫生—环境卫生学]