惠阳胡须鸡禽白血病病毒多样性PCR-HRM技术检测方法的建立与应用  

Development and Application of PCR-HRM Method for Detection of Leukemia Virus Polymorphism in Huiyang Beard Chicken

作　　者：陈圆 陈岸妃 李红伟 张春红 郭鹏举[3] CHEN Yuan;CHEN Anfei;LI Hongwei;ZHANG Chunhong;GUO Pengju(School of Life Science,Huizhou University,Huizhou 516007,Guangdong,China;Guangdong Academy of Medical Sciences,Guangzhou 510640;Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory Animals,Guangzhou 510000,Guangdong,China)

机构地区：[1]惠州学院生命科学学院,广东惠州516007 [2]广东省农业科学院动物卫生研究所,广东广州510640 [3]广东省实验动物监测所广东省实验动物重点实验室,广东广州510000

出　　处：《惠州学院学报》2021年第3期31-34,89,共5页Journal of Huizhou University

基　　金：惠州市科技专项资金(2019X0701012);广东省自然科学特色创新类项目(2018KTSCX217);惠州学院重点培育项目(hzux1201623)。

摘　　要：建立PCR—高分辨率熔解曲线分析体系检测惠阳胡须鸡禽白血病病毒囊膜糖蛋白基因的gp85序列,以期对ALV不同亚型病毒进行分类与鉴定.采用PCR-HRM方法,成功快速检测出400例惠阳胡须鸡样本所携带禽白血病病毒不同亚型,建立了一种快速区分禽白血病内源型和外源型病毒的检测方法,为惠阳胡须鸡国家保种基地对禽白血病的净化提供快速检测.To classify and identify different sub-types of avian leukosis virus(ALV),a PCR-high resolution melting curve analysis system(PCR-HRM)was established to detect the gp85 sequence of envelope glycoprotein gene of ALV of Huiyang bearded chicken.Different sub-types of ALV were detected by PCR-HRM in 400 samples of Huiyang Bearded chickens.A rapid method for distinguishing the endogenous and exogenous sub-types of avian leukosis virus was thus established.It provides a rapid detection for the purification of avian leukemia in Huiyang bearded chicken national breeding base.

关 键 词：PCR-高分辨熔解曲线法 禽白血病 病毒囊膜糖蛋白基因gp85 

分 类 号：S858[农业科学—临床兽医学]