马蓝染色体制片技术优化  

Technology Optimization of the Preparation of Chromosomes in Strobilanthes Cusia

作　　者：赵云青[1,2] 黄颖桢[1,2] 刘保财[1,2] 陈菁瑛[1,2] 张武君[1,2] 俞静 ZHAO Yun-qing;HUANG Ying-zhen;LIU Bao-cai;CHEN Jing-ying;ZHANG Wu-jun;YU Jing(Institute of Agricultural Biological Resources, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;Medicinal Plant Research Center, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China)

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003 [2]福建省农业科学院药用植物研究中心,福建福州350003

出　　处：《福建农业科技》2021年第7期1-9,共9页Fujian Agricultural Science and Technology

基　　金：福建省人民政府、中国农业科学院“5511”协同创新工程(XTCXGC2021003);“一带一路”国家传统草药本底整理及数据库建设(2018FY10070);福建省农业科学院项目(A2017-5);福建省农业科学院十四五创新团队(CXTD2021014-2)。

摘　　要：以莆田地区马蓝分生组织为材料,采用压片技术,探讨马蓝不同取材部位、不同取材时间、不同预处理试剂处理、不同解离时间等对马蓝染色体制片的影响,并进行核型分析。结果表明:以根尖分生组织为材料,在上午8:00、8:30取材,用0.1%秋水仙素溶液室温预处理0.5 h,用卡诺液固定4 h,然后用1 mol·L^(-1)盐酸在60℃水浴8 min,卡宝品红染色8 min,可获得分散良好,形态清晰的染色体。核型分析表明,马蓝染色体为32条,马蓝的核型公式是2x=2n=32=4sm+28m,核型类型为1 B,不对称系数为59.54%。说明莆田地区马蓝在相对稳定的自然环境中进化速度缓慢。By taking the meristem of Strobilanthes cusia cultivated in Putian as the material and using the conventional method,the effects of different sampling sites,sampling time,pretreatment with different reagents and dissociation time on the preparation of chromosomes in Strobilanthes cusia were studied,and the karyotype was analyzed.The results showed that:by taking the root apical meristem as the material,the root apical meristem was sampled at 8:00 and 8:30 a.m.,pretreated with 0.1%colchicine solution at room temperature for 0.5 h,fixed with Carnoy for 4 h,and then bathed with 1 mol·L^(-1) hydrochloric acid at 60℃for 8 min,and stained with carbol fuchsin for 8 min,thus the chromosomes with good dispersion and clear shape could be obtained.The karyotype analysis showed that there were 32 chromosomes in Strobilanthes cusia.The karyotype formula of Strobilanthes cusia was 2x=2n=32=4sm+28m,the karyotype was 1 B,and the asymmetry coefficient was 59.54%.It indicated that the evolution rate of Strobilanthes cusia cultivated in Putian was slow in the relatively stable natural environment.

关 键 词：马蓝 常规制片 染色体 核型分析 

分 类 号：S567.239[农业科学—中草药栽培]