果糖对γ射线照射V79细胞的辐射保护作用  

Radioprotective effect of fructose onγ-irradiated V79 cells

作　　者：胡静[1] 郑越[1] HU Jing;ZHENG Yue(Department of Pharmacy,PLA General Hospital,Beijing 100853,China)

机构地区：[1]解放军总医院医疗保障中心药剂科,北京100853

出　　处：《重庆医学》2021年第17期2885-2888,2893,共5页Chongqing medicine

基　　金：全军医学科技青年培育计划拔尖项目(16QNP079)。

摘　　要：目的探讨果糖对γ射线照射的中国仓鼠肺成纤维细胞(V79细胞)的保护作用及其可能的保护机制。方法根据实验方法将V79细胞分为对照组、辐射组和果糖组,对照组不做特殊处理,辐射组使用辐射处理,果糖组则在辐射组基础上加用果糖处理。采用克隆形成率实验、实验细胞的活力测定(MTS)法和台盼蓝排除法3种方法对果糖的保护作用进行研究,测定超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,用二乙酸二氯荧光素(DCFH-DA)测定活性氧(ROS)水平。结果与辐射组比较,果糖组V79细胞存活率提高,且与暴露于辐射前后的果糖添加时间无关。此外,与辐射组比较,果糖组SOD活性升高,ROS水平降低,差异有统计学意义(P<0.05)。结论果糖具有体外抗辐射活性,其通过提高细胞SOD活性和降低ROS水平来实现细胞保护作用。Objective This study aimed to investigate the protective effect of fructose onγ-ray-irradiated Chinese hamster lung fibroblasts(V79 cells)and its possible protective mechanism.Methods According to the experimental method,V79 cells were divided into the control group,the radiation group and the fructose group.The control group was not treated with special treatment,the radiation group was treated with radiation,and the fructose group was treated with fructose on the basis of the radiation group.Clone formation method,MTS method and trypan blue exclusion method were used to study the protective effect of fructose,the activity of superoxide dismutase(SOD)and catalase(CAT)were measured,the level of reactive oxygen species(ROS)was measured with dichlorofluorescein diacetate(DCFH-DA).Results Compared with the radiation group,the survival rate of V79 cells in the fructose group increased regardless of the fructose addition time before and after exposure to radiation.Besides,compared with the radiation group,the activity of SOD in the fructose group increased,the level of ROS decreased,the difference was statistically significant(P<0.05).Conclusion Fructose has anti-radiation activity in vitro,which achieves cell protection by increasing cell SOD activity and reducing ROS level.

关 键 词：果糖 辐射保护剂 V79细胞 超氧化物歧化酶 过氧化氢酶 

分 类 号：R965.2[医药卫生—药理学]