不同孕期血清LAP分析及参考区间的建立  

Analysis of serum LAP in different trimesters and establishment of reference interval

作　　者：周慧聪 朱艳婷 王子铭 黄梅[1] 严孝岭[1] 虞伟[1] ZHOU Huicong;ZHU Yanting;WANG Ziming;HUANG Mei;YAN Xiaoling;YU Wei(Department of Laboratory Medicine,Taikang Xianlin Drum Tower Hospital Affiliated to Nanjing University Medical School,Medical laboratory,Nanjing,Jiangsu 210046,China)

机构地区：[1]南京大学医学院附属泰康仙林鼓楼医院检验科,南京210046

出　　处：《重庆医学》2021年第17期2917-2919,2924,共4页Chongqing medicine

基　　金：国家自然科学基金项目(81701572);南京大学医学院附属泰康仙林鼓楼医院基金项目(TKKYZD20211609)。

摘　　要：目的分析妊娠妇女与未妊娠妇女血清亮氨酸氨基肽酶(LAP)浓度差异,探讨不同孕期LAP差异并建立不同孕期LAP参考区间。方法选取2018年1月至2019年12月该院就诊孕妇作为研究对象,按不同孕期分早、中、晚孕组,选取同期流产孕妇作为流产组、健康体检妇女作为对照组,比较各组LAP水平,并进行受试者工作特征(ROC)曲线分析、多元logistic回归分析,用Hoffmann法建立参考区间。结果各组LAP水平比较,差异有统计学意义(P<0.05)。ROC曲线下面积为0.989,灵敏度为96.6%,特异度为99.9%,Cut-Off值为67.5 U/L。多元logistic回归结果显示,中孕组OR=1.158(95%CI:1.121~1.195,P<0.05),晚孕组OR=1.197(95%CI:1.159~1.236,P<0.05)。晚孕组比中孕组LAP增长速度更快(P<0.05)。早、中、晚孕组LAP参考区间分别为48.14~194.16、94.68~392.98和108.44~284.86 U/L。结论LAP与妊娠相关性良好,随着孕期的增加而增长。Objective To analyze the difference of serum leucine aminopeptidase(LAP)concentration between pregnant women and non-pregnant women,and discuss the difference of LAP in different trimesters in order to establish the LAP reference interval in different trimesters.Methods The pregnant women who visited the hospital from January 2018 to December 2019 were selected and divided into the early,middle and late pregnancy group according to different pregnancy periods.The pregnant women who had aborted during the same time were selected as the abortion group and the healthy women as the control group,the LAP levels of each group were compared,receiver operating characteristic(ROC)curve and multiple logistic regression were used to analyze,Hoffmann method was used to establish the reference interval.Results There was significant difference in LAP in each group(P<0.05).According to ROC curve,area under the curve was 0.989,the sensitivity was 96.6%,the specificity was 99.9%,Cut-Off point was 67.5 U/L.The results of multiple logistic regression showed that OR=1.158(95%CI:1.121-1.195,P<0.05)in the middle pregnancy group OR=1.197(95%CI:1.159-1.236,P<0.05)in the late pregnancy group.LAP growth rate of late pregnancy group was faster than that of middle pregnancy group(P<0.05).The LAP reference intervals in the early,middle and late pregnancy group was 48.14-194.16,94.68-392.98,108.44-284.86 U/L,respectively.Conclusion LAP has a good correlation with pregnancy and increases with the increase of pregnancy.

关 键 词：血清亮氨酸氨肽酶 早孕 中孕 晚孕 参考区间 

分 类 号：R446.1[医药卫生—诊断学]