岩藻黄素抗D-gal诱导SH-SY5Y细胞衰老作用及机制研究  

Anti-aging Effect and Mechanism of Fucoxanthin on D-gal Induced SH-SY5Y Cells

作　　者：朱敏敏 李云鹏 张淼 李晓双 成敏[3] 周珍琼 刘顺梅[1,4] ZHU Minmin;LI Yunpeng;ZHANG Miao;LI Xiaoshuang;CHENG Min;ZHOU Zhenqiong;LIU Shunmei(School of Bioscience and Technology,Weifang Medical University,Weifang,Shandong,261053,China;Division of Scientific Research Management,Weifang Medical University,Weifang,Shandong,261053,China;School of Basic Medicine Sciences,Weifang Medical University,Weifang,Shandong,261053,China;Experimental Center for Medical Research,Weifang Medical University,Weifang,Shandong,261053,China)

机构地区：[1]潍坊医学院生命科学与技术学院,山东潍坊261053 [2]潍坊医学院科研处,山东潍坊261053 [3]潍坊医学院基础医学院,山东潍坊261053 [4]潍坊医学院医学研究实验中心,山东潍坊261053

出　　处：《广西科学》2021年第3期310-320,共11页Guangxi Sciences

基　　金：国家自然科学基金面上项目(81870237);山东省自然科学基金面上项目(ZR2020MD094,ZR2010DM010);山东省大学生科技创新基金项目(S202010438058)资助。

摘　　要：衰老相关的神经退行性疾病发病率不断增高,严重影响老年人生活质量,为探讨岩藻黄素(Fucoxanthin,FUCO)对神经细胞的抗衰老作用及其机制,采用D-gal诱导人神经母细胞瘤细胞(SH-SY5Y细胞)衰老,然后在细胞培养基中添加FUCO对细胞进行干预,检测其细胞活力、SA-β-半乳糖苷酶活性及细胞内丙二醛(MDA)含量,细胞内自噬体形成及自噬(Autophagy)相关蛋白的表达。研究结果显示,5,10μmol/L FUCO可明显抑制D-gal诱导的细胞衰老,显著提高SH-SY5Y细胞活力(P<0.05),降低SH-SY5Y细胞内SA-β-半乳糖苷酶的活性(P<0.05)和MDA含量(P<0.05),但20μmol/L FUCO的抑制作用不明显(P>0.05)。光学显微镜观察结果显示,5,10,20μmol/L FUCO组细胞内自噬体数量明显比模型组增多;Western blot检测结果显示,与模型组比较,10,20μmol/L FUCO组自噬相关蛋白ATG5表达增多、LC3Ⅱ/LC3Ⅰ增大、p-mTOR/mTOR减小(P<0.05)。表明FUCO具有抗D-gal诱导的SH-SY5Y细胞衰老的作用,其作用机制可能与适度调节自噬途径有关。The increasing incidence of aging-related neurodegenerative diseases has seriously affected the life quality of the elderly.In order to explore the anti-aging effect of fucoxanthin(FUCO)on the nerve cells and its mechanism,D-gal was used to induce the senescence of human neuroblastoma cell(SH-SY5Y cell),and then FUCO was added to the cell culture medium for the intervention.The cell viability,SA-β-galactosidase activity,intracellular malondialdehyde(MDA)content,intracellular autophagosome formation and autophagy-related protein expression were detected.The results showed that 5 and 10μmol/L FUCO significantly inhibited D-gal-induced cell senescence and significantly increased SH-SY5Y cell viability(P<0.05),and reduced the intracellular SA-β-galactosidase activity(P<0.05)and the MDA content(P<0.05)in SH-SY5Y cell.But the anti-aging effect of 20μmol/L fucoxanthin was not significant(P>0.05).The results of optical microscopy showed that the number of intracellular autophagosomes in the cells of the 5,10,and 20μmol/L FUCO group was significantly higher than that of the model group.In addition,the results of western blot showed that compared with the model group,the expression of autophagy-related protein ATG5 increased,LC3Ⅱ/LC3Ⅰincreased and p-mTOR/mTOR decreased in 10,20μmol/L FUCO group(P<0.05).It shows that FUCO has the effect of resisting the senescence of SH-SY5Y cell induced by D-gal,and its mechanism of action may be related to the moderate regulation of autophagy pathway.

关 键 词：岩藻黄素 SH-SY5Y细胞 抗衰老 自噬 D-GAL 

分 类 号：R96[医药卫生—药理学]