野大麦的分子核型分析  

Molecular Karyotype Analysis of Wild Hordeum brevisubulatum

作　　者：冯娜 李景环[1] FENG Na;LI Jinghuan(College of Life Science and Technology,Inner Mongolia Normal University,Hohhot,Inner Mongolia Autonomous Region 010020,China)

机构地区：[1]内蒙古师范大学生命科学与技术学院,呼和浩特010020

出　　处：《种子》2021年第8期15-19,24,共6页Seed

基　　金：内蒙古自治区高等学校科学研究项目(NJZY 18024)教育部项目(29 k 00415)。

摘　　要：以野大麦(Hordeum brevisubulatum L.)为材料,采用荧光原位杂交技术,以5 SrDNA、45 SrDNA及重复序列pAs 1和pSc 119.2为探针,与野大麦染色体标本进行原位杂交,通过观察探针在染色体上标记的位置、标记信号的强弱,对野大麦染色体组进行核型分析。结果表明,5 SrDNA位于第10号和第14号染色体的短臂上;45 SrDNA位于第6号、第10号、第11号、第12号、第13号、第14号染色体的短臂上;pAs 1 DNA的荧光信号大部分出现在染色体的端部,部分出现在着丝粒区域;pSc 119.2的荧光信号出现在第7号和第14号染色体短臂的顶端,第10号染色体一条短臂的顶端,另一条染色体可能顶端缺失导致没有出现pSc 119.2荧光信号。核型公式为2 n=4 x=28=28 m(12 SAT),核型类型属于1 A型,核型不对称系数为57.227%。In this study,the 5 SrDNA,45 SrDNA and repeated sequences including pAs 1 and pSc 119.2 were used as probes to carry out in situ hybridization with chromosomes of wild Hordeum brevisubulatum,and karyotype of wild H.brevisubulatum chromosomes was analyzed by observing the position of the probe on chromosome and the strength of marking signal.The results showed that 5 SrDNA was located on the short arms of chromosome(chr)10 and chr 14,while 45 SrDNA was located on the short arms of chr 6,chr 10,chr 11,chr 12,chr 13 and chr 14.In addition,most of the fluorescence signal of pAs 1 DNA appeared at the end of chromosomes,and some appeared in the centromere region.Meanwhile,the fluorescent signal of pSc 119.2 appeared at the tip of the short arms of chr 7 and chr 14,and also at the tip of a short arm of chr 10.There was no fluorescent signal of pSc 119.2 in other chromosome because of tip missing.The karyotype formula was 2 n=4 x=28=28 m(12 SAT)and the karyotype belonged to type 1 A.The asymmetry coefficient of karyotype was 57.227%.

关 键 词：野大麦 核型分析 荧光原位杂交 

分 类 号：Q943[生物学—植物学] S512.3[农业科学—作物学]