贵紫麦1号紫粒性状的遗传规律及基因定位  

Genetic Development and Gene Mapping of Purple Grain Traits in Guizimai 1

作　　者：董亚兵 何方 彭亚姝 徐如宏[1] 钱小康 任明见[1] DONG Yabing;HE Fang;PENG Yashu;XU Ruhong;QIAN Xiaokang;REN Mingjian(Guizhou University Agriculture College/National Wheat Improvement Center Guizhou Branch,Guiyang 550025,China;Zunyi Agriculture Committee,Zunyi Guizhou 563000,China)

机构地区：[1]贵州大学农学院/国家小麦改良中心贵州分中心,贵阳550025 [2]遵义市农业农村局,贵州遵义563000

出　　处：《种子》2021年第8期57-63,共7页Seed

基　　金：贵州省农业成果转化计划项目(黔科合成果[2019]4246,黔科合平台人才[2017]5788,黔科合基础[2019]1073);国家自然科学基金项目(31660390);国家重点研发计划“七大农作物育种”重点专项(2017 YFD 0100901-4)。

摘　　要：为明确紫色小麦紫粒性状的遗传规律及其调控基因,采用贵紫麦1号(紫粒小麦)×贵农19或贵农麦30(白粒小麦)进行正反交,构建不同杂交世代(F_(1)~F_(5)和BC_(1)F_(1))遗传研究群体,根据杂交后代紫粒性状的分离情况探究其粒色的遗传规律,利用全基因组重测序技术(WGS)结合集群分离分析(BSA)策略对贵紫麦1号紫粒性状进行基因定位。结果表明,贵紫麦1号的紫粒性状受两对显性互补基因控制。基于全基因组重测序及标记筛选结果显示,控制贵紫麦1号紫粒性状的两个互补显性基因分别位于2 AL和7 DL染色体上,并将控制紫粒性状的基因命名为GZMpp1和GZMpp2;紫粒基因GZMpp1与标记chr 2 A 32的遗传距离为1.2 cM;GZMpp2与标记DY-7 D 6的遗传距离为0.5 cM。To clarify the genetic development and regulatory genes of purple grain traits in purple wheat,the reciprocal cross was made between Guizimai 1(purple grain wheat)and Guinong 19 or Guinongmai 30(white grain wheat)to construct research populations with different hybrid generations(F_(1)to F_(5)and BC_(1)F_(1)).According to the separation of purple grain traits of hybrid offspring,the genetic law of grain color was explored.Genome-wide sequencing(GWS)combined with cluster separation analysis(BSA)were used to map purple grain traits in Guizimai 1.The results showed that the purple grain traits of Guizimai 1 were controlled by two pairs of dominant complementary genes.And,the results of GWS and marker screening showed that the two complementary phenotypic motifs controlling purple grain traits of Guizimai 1 were located on 2 AL and 7 DL chromosomes,and the motifs controlling purple grain traits were further named GZMpp1 and GZMpp2.The genetic distance between GZMpp1 and the marker chr2 A 32 was 1.2 cM,while The genetic distance between GZMpp2 and the marker DY-7 D 6 was 0.5 cM.

关 键 词：紫粒小麦 遗传规律 全基因组重测序 基因定位 

分 类 号：S512.1[农业科学—作物学]