不同冬青品种对盐胁迫的生长及生理响应  

Response of Salt Stress on Growth and Physiological of Ilex Cultivars

作　　者：梁春飞 石冠旗 金晓玲 LIANG Chunfei;SHI Guanqi;JIN Xiaoling(Xingjian College of Science and Liberal Arts,Guangxi University,Nanning,Guangxi 530000;College of Landscape Architecture,Central South University of Forestry and Technology,Changsha,Hunan 410004;Forestry Bureau of Lingling District,Yongzhou,Hunan 425000)

机构地区：[1]广西大学行健文理学院,广西南宁530000 [2]中南林业科技大学风景园林学院,湖南长沙410004 [3]永州市零陵区林业局,湖南永州425000

出　　处：《北方园艺》2021年第16期74-82,共9页Northern Horticulture

基　　金：2020年度广西高等教育本科教学改革工程资助项目(2020JGA375);国家林草局948资助项目(2008-4-14)。

摘　　要：以7个冬青品种为试材,采用温室控盆法,研究了不同浓度NaCl((0、3.0、6.0、9.0 g·L^(-1))胁迫对其生长和生理影响,并运用隶属函数法综合评价冬青品种NaCl胁迫下的耐受性强弱,以期为冬青品种适生区域的确定提供参考依据。结果表明:1)冬青的盐害症状、死亡率和株高生长与胁迫天数和胁迫浓度显著相关;品种之间存在显著差异。2)相对电导率(Rce)和丙二醛(MDA)含量均与胁迫天数和胁迫浓度呈正相关;超氧化物歧化酶(SOD)活性和可溶性蛋白质含量均与胁迫天数和浓度呈负相关;而过氧化物酶(POD)活性除‘Blue Maid’,与胁迫天数呈正相关外,其它6个品种均随着胁迫天数的延长和胁迫浓度的提高呈先上升后下降的趋势;叶绿素含量(SPAD)则随着胁迫天数的延长呈逐渐下降的趋势。3)叶片盐害指数、Rec、MDA、可溶性蛋白质含量可作为冬青品种耐NaCl胁迫强弱的综合评价指标。4)隶属函数法综合评价结果,耐NaCl胁迫的强弱为‘Blue Maid’>‘Emily Bruner’>‘Tensaw’>‘Anna′s Choice’>‘Mary Nell’>‘Savannah’>‘Forstery’。Seven Ilex cultivars were used as test materials.The influence of different concentrations of NaCl(0 g·L^(-1),3.0 g·L^(-1),6.0 g·L^(-1)and 9.0 g·L^(-1))for 50 days on the growth and physiological characteristics of two-year-old cuttage seedlings of seven Ilex cultivars were studied by pot culture in the plastic greenhouse,and membership function method was used to evaluate the tolerance of Ilex cultivars under NaCl stress,in order to provide a reference for determining the suitable area of the holly variety.The results showed that,1)Symptom,mortality and plant height growth of salt injury to Ilex cultivars were significantly correlated with stress duration and stress intensity.There were significant differences among Ilex cultivars.2)The Rec and MDA increased with the stress time and the stress concentration.SOD activity and soluble protein content increased at first and then decreased with the extension of stress days.The activity of POD increased at first and then decreased in the other six cultivars except‘Blue Maid’which increased continuously with the extension of stress days.SPAD decreased gradually with the extension of stress.3)The contribution rate and weight value of leaf salt injury index,Rec,MDA,soluble protein content and SPAD were higher,which could be used as the comprehensive evaluation index for the identification of seven Ilex cultivars resistant to NaCl stress.4)The comprehensive evaluation results of the membership function method showed that the degree of resistance to NaCl stress was as follows,‘Blue Maid’>‘Emily Bruner’>‘Tensaw’>‘Anna′s Choice’>‘Mary Nell’>‘Savannah’>‘Forstery’.

关 键 词：冬青品种 盐害指数 相对电导率 丙二醛含量 可溶性蛋白质含量 叶绿素含量 

分 类 号：S686[农业科学—观赏园艺]