海岛棉GH9基因家族成员鉴定及分析  

Genome-Wide Identification and Analysis of GH 9 Gene Family in Gossypium barbadense L.

作　　者：刘正文 王省芬[1] 孟成生[1] 张艳[1] 孙正文 吴立强[1] 马峙英[1] 张桂寅[1] LIU Zhengwen;WANG Xingfen;MENG Chengsheng;ZHANG Yan;SUN Zhengwen;WU Liqiang;MA Zhiying;ZHANG Guiyin(State Key Laboratory of North China Crop Improvement and Regulation,Key Laboratory for Crop Germplasm Resources of Hebei,College of Agronomy,Hebei Agricultural University,Hebei Baoding 071000,China)

机构地区：[1]河北农业大学农学院,华北作物改良与调控国家重点实验室,河北省作物种质资源重点实验室,河北保定071000

出　　处：《中国农业科技导报》2021年第9期30-45,共16页Journal of Agricultural Science and Technology

基　　金：国家重点研发计划项目(2018YFD0100304)。

摘　　要：植物GH 9基因在细胞壁的生物合成和重塑中起着重要作用,对海岛棉GH 9基因进行全基因组鉴定与分析,挖掘其在棉纤维发育中的作用。从海岛棉全基因组中鉴定到53个GH 9基因,分析其理化性质、基因结构、染色体分布、进化历程、棉纤维发育过程中表达模式及转录调控。结果表明,53个GbGH 9 s分为A、B、C三组,定位在22条染色体上;多倍化事件是海岛棉GH 9基因家族扩张的主要驱动力,基因复制后经历严格的选择约束;A组多个成员在棉纤维发育整个时期尤其是次生壁加厚期高表达,C组多个成员在棉纤维起始和伸长期优势表达,B组具有相对复杂的表达模式,生长素和乙烯可能在GbGH 9 s的转录调控中发挥重要作用。此外,通过回交将GbGH 9B6导入陆地棉可提高棉纤维强度。海岛棉GH 9基因家族成员的鉴定及分析有助于明确其进化和功能,为后续研究提供重要参考。GH 9 genes play vital roles in the biosynthesis and remodeling of plant cell walls.The aim of this study was to discover fiber development-related GH 9 genes by the identification and analysis of this gene family in Gossypium barbadense.A total of 53 GH 9 genes were identified from Gossypium barbadense,and their physico-chemical properties,gene structure,chromosomal distribution and evolutionary history were analyzed.This paper also investigated the expression patterns and transcriptional regulation of GbGH 9 s during cotton fiber development.As a result,53 GbGH 9 s were divided into three groups(A,B and C)and were distributed on 22 chromosomes.The expansion of GH 9 gene family was mainly driven by polyploidization in Gossypium barbadense,and the duplicated genes had been under strong selective constraints.Some members of group A showed high expression levels during cotton fiber development,especially during secondary wall thickening.Some members of group C were highly expressed during cotton fiber initiation and elongation.In addition,the expression patterns of group B were relatively complex.Auxin and ethylene may have played important roles in the transcriptional regulation of GbGH 9 s.It improved cotton fiber strength by the introgression of GbGH 9B6 into Gossypium hirsutum.The identification and analysis of GbGH 9 s were critical for understanding their evolution and functions,and provided a support for future studies.

关 键 词：海岛棉 GH 9基因家族 进化 表达分析 棉纤维发育 

分 类 号：Q78[生物学—分子生物学] S562[农业科学—作物学]