新疆地区FGB基因多态性对纤维蛋白原水平及胆囊结石发病的影响  

Effects of FGB gene polymorphism on fibrinogen level and gallstones incidence in Xinjiang,China

作　　者：阿丽叶古丽·艾皮热[1] 皮尔地瓦斯·麦麦提玉素甫[2] 王俭[2] 艾克拜尔·艾力[1,2] 克力木·阿不都热依木[1,2] ALIYEGULI Aipire;PIERDIWASI Maimaitiyusufu;WANG Jian;AIKEBAIER Aili;KELIMU Abudureyimu(Institute of Minimally Invasive Surgery,The People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China;Department of Minimally Invasive,Hernia,and Abdominal Wall Surgery,The People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院普外微创研究所,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院微创、疝和腹壁外科,乌鲁木齐830001

出　　处：《临床肝胆病杂志》2021年第9期2171-2176,共6页Journal of Clinical Hepatology

基　　金：新疆维吾尔自治区自然科学基金项目(2017D01C096)。

摘　　要：目的探讨纤维蛋白原β链(FGB)基因rs4220、rs1044291位点多态性与血浆纤维蛋白原(Fg)水平及胆囊结石的相关性。方法收集2017年12月—2020年5月在新疆维吾尔自治区人民医院住院治疗及健康体检的维吾尔族、汉族胆囊结石及非胆囊结石患者各150例,采集血液标本,测定血浆Fg水平,并在前期的FGB基因全外显子测序结果的基础上,选择FGB基因rs4220、rs1044291多态位点,采用SNaPshot法进行基因型鉴定。符合正态分布的计量资料2组间比较采用t检验,非正态分布的计量资料2组间比较采用Mann-Whitney U检验,计数资料组间比较采用χ2检验;各变量与胆囊结石的关系采用多因素logistic回归分析。结果在汉族人群中,胆囊结石组血浆Fg水平高于对照组,差异有统计学意义[2.90(2.43~3.49)g/L vs 2.47(2.06~3.02)g/L,Z=-3.62,P<0.01],而维吾尔族人群中无差异。FGB基因rs4220、rs1044291位点基因频率在维吾尔族、汉族胆囊结石组与对照组差异均无统计学意义(P值均>0.05);在汉族人群中,胆囊结石组rs4220位点GG基因型者血浆Fg水平高于对照组[2.84(2.32~3.61)g/L vs 2.34(2.05~2.75)g/L,Z=-3.04,P<0.05],rs1044291位点T基因型携带者Fg水平高于对照组[(3.08±0.75)g/L vs(2.48±0.48)g/L,t=2.80,P<0.05)]。在维吾尔族人群中,胆囊结石组与对照组间比较,仅有rs4220位点的A基因携带者血浆Fg水平低于对照组[2.84(2.08~3.06)g/L vs 3.10(2.85~3.98)g/L,Z=-2.41,P<0.05]。但两个民族胆囊结石组、对照组内,各基因型携带者Fg水平差异均无统计学意义(P值均>0.05)。结论FGB基因多态性对血浆Fg水平的影响可能与种族有关;FGB基因rs4220、rs1044291位点多态性可能通过调控Fg水平影响新疆人群胆囊结石的发病。Objective To investigate the association of the polymorphisms of the FGB gene rs4220 and rs1044291 loci with plasma fibrinogen(Fg)level and gallstones in Xinjiang,China.Methods Blood samples were collected from 150 Uygur and Han patients with gallstones and 150 Uygur and Han individuals without gallstones who were hospitalized or underwent physical examination in The People’s Hospital of Xinjiang Uygur Autonomous Region from December 2017 to May 2020.Plasma Fg level was measured,and based on the previous results of whole exon sequencing of the FGB gene,the SNaPshot method was used to identify the genotype at rs4220 and rs1044291 loci of the FGB gene.The t-test was used for comparison of normally distributed continuous data between two groups,and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups;the chi-square test was used for comparison of categorical data between two groups;a multivariate logistic regression analysis was used to investigate the association between each variable and gallstones.Results For the Chinese Han population,the gallstones group had a significantly higher plasma Fg level than the control group[2.90(2.43-3.49)g/L vs 2.47(2.06-3.02)g/L,Z=-3.62,P<0.01),while there was no significant difference in the Uyghur population.There were no significant differences in the frequencies of genotypes and alleles at the rs4220 and rs1044291 loci of the FGB gene between the gallstones group and the control group in both Uyghur and Han populations(all P>0.05).For the Chinese Han population,the subjects carrying GG genotype at the rs4220 locus in the gallstones group had a significantly higher plasma Fg level than those in the control group[2.84(2.32-3.61)g/L vs 2.34(2.05-2.75)g/L,Z=-3.04,P<0.05],and the subjects carrying T genotype at the rs1044291 locus had a significantly higher plasma Fg level than those in the control group(3.08±0.75 g/L vs 2.48±0.48 g/L,t=2.80,P<0.05).For the Uyghur population,only the subjects carrying A genotype at the r

关 键 词：胆囊结石病 纤维蛋白原 多态现象 遗传 新疆[维吾尔自治区] 

分 类 号：R657.42[医药卫生—外科学]