秦皮苷减轻LPS诱导的肺炎小鼠肺组织炎症和氧化损伤  

Fraxin attenuates lung inflammation and oxidative damage in mice with pneumonia induced by LPS

作　　者：徐宜全[1] 蔡忠贵 潘延成[3] XU Yiquan;CAI Zhonggui;PAN Yancheng(Department of Respiratory Medicine,Tengzhou Hospital of Traditional Chinese Medicine,Tengzhou 277599,China;Department of Internal Medicine,Zaozhuang Hospital,Zaozhuang Mining Group,Zaozhuang 277100,China;Department of Pharmacy,Tengzhou Central People's Hospital,Tengzhou 277599,China)

机构地区：[1]滕州市中医医院呼吸内科,277599 [2]枣庄矿业集团枣庄医院内科,277100 [3]滕州市中心人民医院药房,277599

出　　处：《免疫学杂志》2021年第9期809-815,共7页Immunological Journal

摘　　要：目的研究秦皮苷(fraxin)对脂多糖(lipopolysaccharides,LPS)诱导的肺炎小鼠肺组织炎症和氧化损伤的影响。方法实验分成Control(空白对照)、Model(肺炎模型)、fraxin-A(肺炎模型,8 mg/kg秦皮甙灌胃)、fraxin-B(肺炎模型,16 mg/kg秦皮甙灌胃治疗)、fraxin-C组(肺炎模型,32 mg/kg秦皮甙灌胃)。取肺组织和肺泡灌洗液,检测肺组织湿/干重比例,计数肺泡灌洗液细胞总数和中性粒细胞数目;ELISA法检测肺泡灌洗液中白细胞介素-6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-10(interleukin-10,IL-10)含量;比色法检测肺泡灌洗液中超氧化物歧化酶(superoxide orgotein dismutase,SOD)和一氧化氮(nitric oxide,NO)含量;可见分光光度法检测肺泡灌洗液中丙二醛(malonaldehyde,MDA)含量;Western blot法检测肺组织中p-NF-κBp65、核转录因子-κB(nuclear factor-kappaB,NF-κB)p65、Toll样受体4(Toll-like receptor-4,TLR4)、诱导型一氧化氮合成酶(inducible nitric oxide synthase,iNOS)、p-IκBα、IκBα蛋白表达。结果与Control组比较,Model组肺组织湿/干重比例升高,肺泡灌洗液细胞总数和中性粒细胞数目增多,肺泡灌洗液中IL-6、IL-1β、TNF-α含量和MDA、NO含量升高,SOD含量下降,肺组织中NF-κBp65、TLR4、i NOS蛋白水平升高。与Model组比较,fraxin-A、fraxin-B、fraxin-C组肺组织湿/干重比例降低,肺泡灌洗液细胞总数和中性粒细胞数目减少,肺泡灌洗液中IL-6、IL-1β、TNF-α含量和MDA、NO含量降低,SOD含量升高,肺组织中NF-κBp65、TLR4、iNOS蛋白水平降低。结论秦皮苷减轻LPS诱导的肺炎小鼠肺组织炎症和氧化损伤,其机制可能与TLR4/NF-κB有关。This study was performed to investigate the effect of fraxin on lung tissue inflammation and oxidative damage in mice with lipopolysaccharides(LPS)-indued pneumonia.Mice were divided into control(blank control),model(pneumonia model),fraxin-A(pneumonia model,8 mg/kg fraxin gavage),fraxin-B(pneumonia model,16 mg/kg fraxin gavage),and fraxin-C(pneumonia model,32 mg/kg fraxin gavage).Lung tissue and alveolar lavage fluid were collected for detecting the wet/dry weight ratio of lung tissue,and counting the total number of alveolar lavage fluid cells and the number of neutrophils,the content of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),interleukin-10(IL-10)in alveolar lavage fluid was detected by ELISA.Colorimetric method was used to detect the content of superoxide orgotein dismutase(SOD)and nitric oxide(NO)in alveolar lavage fluid;visible spectrophotometry was used to detect the content of malonaldehyde(MDA)in alveolar lavage fluid;Western blot was used to detect theexpression of p-NF-κBp65,nuclear factor-kappa Bp65(NF-κBp65),Toll-like receptor-4(TLR4),p-IκBα,IκBα,and i NOS proteins in lung tissues Datashowed that compared with the control group,the wet/dry weight ratio of lung tissue in the model group was increased,and the total number of cells,the number ofneutrophils and the levels of IL-6,IL-1β,TNF-α,MDA and NO in the alveolar lavage fluid were increased,butSOD level decreased,while the levels of NF-κBp65,TLR4 and i NOS proteins in lung tissue increased.Fraxin couldreverse the changes mentioned above in the groups of fraxin-A,fraxin-B,and fraxin-C.In conclusion,fraxin canreduce lung tissue inflammation and oxidative damage in LPS-pneumonia mice,and its mechanism may related toTLR4/NF-κB.

关 键 词：秦皮苷 肺炎 炎症 氧化损伤 

分 类 号：R563.1[医药卫生—呼吸系统]