裂隙核细胞及外周血T淋巴细胞亚群在百日咳患儿中的表达探究  

Investigation on the Expression of Cleaved Lymphocyte and Peripheral Blood T Lymphocyte Subsets in Children with Pertussis

作　　者：吴少珍[1] 杨海霞[1] 黄柏开[1] 邓坤仪[1] 黄道连[1] 童辉纯 WU Shaozhen;YANG Haixia;HUANG Bokai;DENG Kunyi;HUANG Daolian;TONG Huichun(Boai Hospital of Zhongshan,Zhongshan 528403,China)

机构地区：[1]广东省中山市博爱医院,广东中山528403

出　　处：《中国医学创新》2021年第23期131-135,共5页Medical Innovation of China

基　　金：中山市社会公益与基础研究项目(2020B1067)。

摘　　要：目的:探究裂隙核细胞及外周血T淋巴细胞亚群在百日咳患儿中的表达情况。方法:选取本院2019年1月-2020年9月的80例百日咳患儿为观察组,同时期的80名体检健康儿童为对照组。比较两组的裂隙核细胞阳性率及构成、外周血T淋巴细胞亚群指标(CD3^(+)、CD4^(+)、CD8^(+)及CD4^(+)/CD8^(+)),并比较观察组中不同严重程度(轻症、重症)及疾病分期(卡他期、痉咳期及恢复期)患儿的裂隙核细胞阳性率及构成、外周血T淋巴细胞亚群指标,采用Spearman相关性分析裂隙核细胞、外周血T淋巴细胞亚群指标与百日咳严重程度的关系。结果:观察组裂隙核细胞阳性率及构成均显著高于对照组,差异均有统计学意义(P<0.05)。观察组中重症患儿裂隙核细胞阳性率及构成均高于轻症患儿,差异均有统计学意义(P<0.05)。观察组中不同疾病分期患儿的裂隙核细胞阳性率及构成情况比较,差异均有统计学意义(P<0.05)。观察组中卡他期和恢复期、痉咳期和恢复期患儿裂隙核细胞阳性率及构成情况比较,差异均有统计学意义(P<0.05)。观察组CD8^(+)显著高于对照组,CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)均显著低于对照组,差异均有统计学意义(P<0.05)。观察组中重症患儿CD8^(+)高于轻症患儿,CD3^(+)、CD4^(+)及CD4^(+)/CD8^(+)均低于轻症患儿,差异均有统计学意义(P<0.05)。观察组中不同疾病分期患儿外周血T淋巴细胞亚群指标比较,差异均有统计学意义(P<0.05)。卡他期和痉咳期、卡他期和恢复期、痉咳期和恢复期的外周血T淋巴细胞亚群指标比较,差异均有统计学意义(P<0.05)。Spearman相关性分析显示,裂隙核细胞及外周血T淋巴细胞亚群指标均与百日咳严重程度有密切关系(P<0.05)。结论:裂隙核细胞及外周血T淋巴细胞亚群在百日咳患儿中的检测价值较高,对于疾病的严重程度及疾病分期均有一定的反应意义。Objective:To investigate the expression of cleaved lymphocytes and peripheral blood T lymphocyte subsets in children with pertussis.Method:A total of 80 children with pertussis in our hospital from January 2019 to September 2020 were selected as the observation group,and 80 healthy children in the same period were selected as the control group.The positive rate and composition of cleaved lymphocytes and peripheral blood T lymphocyte subsets (CD3^(+),CD4^(+),CD8^(+) and CD4^(+)/CD8^(+)) in the two groups were compared,and the positive rate and composition of cleaved lymphocytes and peripheral blood T lymphocyte subsets in children with different severity (mild and severe) and disease stage (catarrhal stage,spasmodic cough stage and recovery stage) in the observation group were compared,Spearman correlation was used to analyze the relationship between cleaved lymphocytes,peripheral blood T lymphocyte subsets and the severity of pertussis.Result:The positive rate and composition of cleaved lymphocytes in the observation group were significantly higher than those in the control group,the differences were statistically significant (P<0.05).The positive rate and composition of cleaved lymphocytes in severe children in the observation group were higher than those in mild children,the differences were statistically significant (P<0.05).Comparison of the positive rate and composition of cleaved lymphocytes in children with different disease stages in the observation group,the differences were statistically significant (P<0.05).The positive rate and composition of cleaved lymphocytes in the observation group were compared between catarrhal stage and recovery stage,and between spasmotic cough stage and recovery stage,the differences were statistically significant (P<0.05).CD8^(+) in the observation group was significantly higher than that in the control group,CD3^(+),CD4^(+) and CD4^(+)/CD8^(+) were significantly lower than those in the control group,the differences were statistically significant (P<0.05).In the observation

关 键 词：裂隙核细胞 外周血T淋巴细胞亚群 百日咳 

分 类 号：R725.1[医药卫生—儿科]