油莎豆种质资源遗传多样性的RAPD分析  

RAPD Analysis on Genetic Diversity of Cyperus esculentus Germplasm Resources

作　　者：魏尊苗 王占海[1] 杨向东[2] 牛陆[2] 程艳[1] 刘佳遥 牟忠生[1] Wei Zunmiao;Wang Zhanhai;Yang Xiangdong;Niu Lu;Cheng Yan;Liu Jiayao;Mu Zhongsheng(Economic Plant Research Institute,Jilin Academy of Agricultural Sciences,Changchun,130033;Agricultural Biotechnology Research Institute,Jilin Academy of Agricultural Sciences,Changchun,130033)

机构地区：[1]吉林省农业科学院经济植物研究所,长春130033 [2]吉林省农业科学院农业生物技术研究所,长春130033

出　　处：《分子植物育种》2021年第16期5428-5434,共7页Molecular Plant Breeding

基　　金：吉林省重大科技专项(192480NY010166906);国家重点研发计划项目(2019YFD1002603);吉林省农业科技创新工程项目(CXGC2019DC007)共同资助。

摘　　要：本研究利用RAPD分子标记技术对19份油莎豆资源和1份香附子资源进行遗传多样性分析,从96个引物中筛选出24个条带清晰且重复性好的引物,扩增得到134个位点,其中94个位点具有多态性,多态性率为70.15%。NTSYS聚类分析显示,供试油莎豆与香附子遗传相似系数的变化幅度0.41~0.46,供试油莎豆材料间遗传相似系数的变化幅度为0.92~1.00,以遗传相似系数0.40为阈值,可将供试油莎豆与香附子聚成两大类。结果表明,供试油莎豆材料之间相似性较高,遗传基础相近,且油莎豆材料地域性不明显;莎草属种间遗传多样性丰富。RAPD分析结果为后续研究莎草族种质间的遗传多样性提供理论依据。The research analyzed genetic diversity of nineteen tiger nuts and one Cyperus rotundus with RAPD molecular markers. 24 clear and repeatable primers were selected from 96 primers, 94 polymorphic locus were found from 134 locus amplified by selected primers, the ratio of polymorphic locus was 70.15%. Cluster analysis showed that the genetic similarity coefficient was ranged from 0.41~0.46 between tested tiger nut and Cyperus rotundus;The genetic similarity coefficient among all tiger nuts was range from 0.92~1.00, tested materials were divided into two groups at the similarity coefficient of 0.40. The result showed that there was high similarity between tested tiger nut materials, they had similar genetic basis, and the seeds from the same region were not gathered into one group;the genetic diversity of Cypereae Linn germplasm interspecies was rich. The results of RAPD analysis provide theoretical basis for the subsequent study of genetic diversity among Cypereae Linn germplasm.

关 键 词：油莎豆(Cyperus esculentus) 种质资源 RAPD分析 遗传多样性 

分 类 号：S565.9[农业科学—作物学]