牛角地黄汤对免疫性血小板减少症大鼠外周血单个核细胞IDO表达的影响  

Effects of Niujiao Dihuang Decoction on IDO Expression in Peripheral Blood Mononuclear Cells of Immune Thrombocytopenia Rats

作　　者：聂甜[1] 江劲波[1] 何娅娜[1] 杨琳[1] 周欣欣[1] 郝敬全[1] 刘凯[1] 宁彩虹 NIE Tian;JIANG Jingbo;HE Yana;YANG Lin;ZHOU Xinxin;HAO Jingquan;LIU Kai;NING Caihong(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2021年第9期1334-1338,共5页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅科学研究项目(18C0358);湖南中医药大学护理学一级学科开放基金项目(2019HLX07)。

摘　　要：目的探讨牛角地黄汤对免疫性血小板减少症(ITP)大鼠外周血单个核细胞吲哚胺2,3-双加氧酶(IDO)表达的影响及其作用机制。方法将36只SD大鼠用兔抗SD大鼠血小板血清法建立ITP大鼠模型后随机分成模型组、阳性对照组、实验组,另设空白组,每组12只。于造模第7天,实验组、阳性对照组分别予牛角地黄汤、地塞米松干预,模型组和空白组给予等体积的生理盐水干预。4周后处死各组大鼠,用RT-PCR检测外周血单个核细胞IDO、JAK1、STAT1 mRNA表达水平,Western blot检测外周血单个核细胞IDO蛋白表达量。结果与空白组比较,模型组ITP大鼠外周血单个核细胞IDO蛋白和IDO、JKA1、STAT1 mRNA表达水平均降低(P<0.05)。与模型组比较,实验组和阳性对照组ITP大鼠外周血单个核细胞IDO、JAK1、STAT1 mRNA和IDO蛋白表达均增高(P<0.05)。结论牛角地黄汤可能通过JAK1/STAT1信号分子促进ITP大鼠外周血单个核细胞IDO表达,减少血小板免疫性损伤。Objective To investigate the effect and possible mechanism of Niujiao Dihuang Decoction on indoleamine 2,3-dioxygenase(IDO)expression in peripheral blood mononuclear cells of immune thrombocytopenia(ITP)rats.Methods ITP rat model was established by rabbit anti SD rat platelet serum method in 36 SD rats,and were randomly divided into model group,positive control group and experimental group,with a blank group,12 rats in each group.On the 7th day of modeling,rats in the experimental group and positive control group were respectively given Niujiao Dihuang Decoction and dexamethasone intervention,and rats in the model group and blank group were given the same volume of normal saline intervention.Rats in each group were sacrificed 4 weeks later.The mRNA expression levels of IDO,JAK1,STAT1 in peripheral blood mononuclear cells were detected by RT-PCR,and the protein expression level of IDO in peripheral blood mononuclear cells was detected by Western blot.Results Compared with blank group,IDO protein and mRNA expression levels of IDO,JKA1 and STAT1 in peripheral blood mononuclear cells of ITP rats in model group were decreased(P<0.05).Compared with model group,the mRNA expression levels of IDO,JAK1,STAT1 and IDO protein expression in peripheral blood mononuclear cells of ITP rats in experimental group and positive control group were significantly increased(P<0.05).Conclusion Niujiao Dihuang Decoction may promote the expression of IDO in peripheral blood mononuclear cells of ITP rats through JAK1/STAT1 signaling molecule and reduce platelet immune injury.

关 键 词：免疫性血小板减少症 外周血单个核细胞 牛角地黄汤 吲哚胺2 3-双加氧酶 JAK1 SATA1 

分 类 号：R255.9[医药卫生—中医内科学]