Rapid Internal Control Reference Recombinase-Aided Amplification Assays for EBV and CMV Detection  

作　　者：GAO Yuan TIE Yan Qing ZHAO Lin Qing TAN He DING Nan DING Ya Xin GUO Qi ZHANG Rui Qing WANG Jin Rong CHEN Zi Wei FAN Guo Hao SHEN Xin Xin FENG Zhi Shan MA Xue Jun 

机构地区：[1]Hebei Medical University,Shijiazhuang 050031,Hebei,China [2]NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China [3]Hebei General Hospital,Shijiazhuang 050070,Hebei,China [4]Laboratory of Virology,Beijing Key Laboratory of Etiology of Viral Diseases in Children,Capital Institute of Pediatrics,Beijing 100020,China [5]The Third Xiangya Hospital of Central South University,Changsha 410013,Hunan,China [6]Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430071,Hubei China

出　　处：《Biomedical and Environmental Sciences》2021年第8期650-655,共6页生物医学与环境科学（英文版）

基　　金：the China Mega-Projects for Infectious Disease[grant no.2018ZX10711001,2017ZX10104001 and 2018ZX10713-002];the National Institute for Viral Disease Control and Prevention[grant no.2019HYDQNJJ03].

摘　　要：Epstein-Barr virus(EBV)and cytomegalovirus(CMV),two of the most prevalent human herpesviruses,cause a wide spectrum of diseases and symptoms and are associated with serious health problem.In this study,we developed an internal control reference recombinase-aided amplification(ICR-RAA)assay for the rapid detection of EBV and CMV within 30 min.The assay had a sensitivity of 5 and 1 copies/test for EBV and CMV,respectively,with no cross reaction with other pathogens.In comparison with those of the commercial quantitative polymerase chain reaction(qPCR),the sensitivity of the EBV and CMV ICR-RAAs using extracted DNA was 93.33%and 84.84%,respectively;the specificity was 98.75%and 100.00%,respectively;and the Kappa values were 0.930 and 0.892(P<0.05),respectively.In comparison with those of qPCR,the sensitivity of the EBV and CMV ICR-RAAs using the DNA by thermal lysis was 72.22%and 80.00%,respectively;the specificity was 100.00%。

关 键 词：SPECIFICITY Internal EBV 

分 类 号：R440[医药卫生—诊断学]