核转录共抑制因子、转化生长因子β1、Smad4在重度宫腔粘连内膜组织中表达及临床意义  

Expression and clinical significance of SnoN,TGF-β1 and Smad4 in endometrium of patients with severe intrauterine adhesion

作　　者：杨博萍[1] 郭建新[2] 王昊 袁艺萍 池余刚[1] 李力[2] YANG Boping;GUO Jianxin;WANG Hao;YUAN Yiping;CHI Yugang;LI Li(Department of Gynecology,Chongqing Health Center for Women and Children,Chongqing,401147;Department of Obstetrics and Gynecology,Daping Hospital,Army Medical University(Third Military Medical University),Chongqing,400042,China)

机构地区：[1]重庆市妇幼保健院普通妇科,重庆401147 [2]陆军军医大学(第三军医大学)大坪医院妇产科,重庆400042

出　　处：《第三军医大学学报》2021年第17期1667-1672,共6页Journal of Third Military Medical University

基　　金：重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0403);重庆市妇幼保健院院内课题面上项目(2019YJMS03)。

摘　　要：目的探讨核转录共抑制因子(ski-related novel protein N,SnoN)在宫腔粘连(intrauterine adhesions,IUA)发病中的作用,及其与转化生长因子β1(transforming growth factor-β1,TGF-β1)、Smad4关系。方法收集重度宫腔粘连(sIUA组)和宫腔无病变的子宫纵隔患者(对照组)宫内膜组织各20例,通过免疫组化、Western blot、RT-qPCR检测子宫内膜组织中SnoN、TGF-β1、Smad4的表达水平,并应用Spearman等级相关分析SnoN、TGF-β1、Smad4之间的相关性,探讨其与宫腔粘连发病的关系。结果sIUA组患者宫内膜组织中TGF-β1、Smad4表达水平明显高于对照组(P<0.01),而SnoN表达水平明显低于对照组(P<0.01)。在重度宫腔粘连宫内膜组织中,TGF-β1与Smad4的蛋白表达水平呈正相关(r=0.4689,P<0.05),而TGF-β1与SnoN的蛋白水平呈负相关(r=-0.4835,P<0.05)。结论SnoN可能通过TGF-β1/Smad4信号通路参与宫腔粘连的发病。Objective To investigate the role of ski-related novel protein N(SnoN)in the pathogenesis of intrauterine adhesion(IUA)and its association with transforming growth factor-β1(TGF-β1)and Smad4 in endometrial tissue in IUA patients.Methods Endometrial tissues of 20 severe IUA patients(sIUA group)and 20 healthy women with uterine septum(control group)were collected from our 2 departments during January 2019 and October 2020.Immunohistochemical assay,Western blotting and reverse transcription quantitative polymerase chain reaction(RT-qPCR)were used to detect the expression of SnoN,TGF-β1 and Smad4 in the endometrial tissue of the control and sIUA groups.The correlations of their expression levels in the endometrial tissues were analyzed with Spearman rank correlation analysis to investigate the association of the levels and IUA.Results The expression levels of TGF-β1 and Smad4 were significantly higher,while that of SnoN was obviously lower in the sIUA group than the control group(all P<0.01).In the endometrial tissues from the sIUA group,the expression of Smad4 was positively correlated with that of TGF-β1(r=0.4689,P<0.05).However,the TGF-β1 expression was negatively correlated with that of SnoN(r=-0.4835,P<0.05).Conclusion SnoN may be involved in the pathogenesis of IUA via the TGF-β1/Smad4 signal pathway.

关 键 词：核转录共抑制因子 转化生长因子β1/Smad4 重度宫腔粘连 

分 类 号：R341[医药卫生—基础医学] R446.6