肺癌组织中长链非编码RNA-H19的表达与患者免疫功能、炎症反应因子及预后的关系  

Relationship between expression of long-chain non-coding RNA-H19 and immune function,inflammatory factors and clinical prognosis in lung cancer patients

作　　者：周建英[1] 刘震天[1] 胡珍珍[1] 肖丹[1] ZHOU Jian-Ying;LIU Zhen-Tian;HU Zhen-Zhen;XIAO Dan(Jiangxi Cancer Hospital,Nanchang 330029,China)

机构地区：[1]江西省肿瘤医院,南昌330029

出　　处：《中国免疫学杂志》2021年第16期2012-2016,共5页Chinese Journal of Immunology

摘　　要：目的:探讨肺癌组织中长链非编码RNA-H19的表达与患者免疫功能、炎症因子以及临床预后的关系。方法:纳入接受手术治疗的80例肺癌患者,q RT-PCR法检测肺癌及癌旁组织中Lnc RNA-H19的表达;免疫比浊法检测患者血清免疫球蛋白;流式检测T细胞亚群;ELISA法检测炎症反应因子的表达。比较Lnc RNA-H19的表达及其与临床病理特征、免疫功能指标、炎症反应因子以及预后的相关性。结果:Lnc RNA-H19在肺癌组织中的相对表达量明显高于癌旁组织(P<0.05);Ln‐c RNA-H19的表达与肿瘤的大小、分化程度、TNM分期以及淋巴结是否转移有关(P<0.05);肺癌组织中Lnc RNA-H19的表达与患者血清免疫功能指标Ig A、Ig G、Ig M、CD3^(+)、CD4^(+)、CD8^(+)呈负相关(r<0,P<0.05);与炎症反应因子IL-1β、IL-6和TNF-α的表达呈正相关(r>0,P<0.05);Lnc RNA-H19高表达组肺癌患者常伴随着免疫功能的下降和机体炎症反应的加重。Lnc RNA-H19高表达组患者术后生存率较Lnc RNA-H19低表达组显著降低(P<0.05)。单因素结果显示:Lnc RNA-H19、肿瘤分化程度、淋巴结是否转移以及肿瘤分期与肺癌患者的术后总生存期有关(P<0.05);Cox多因素结果显示:Lnc RNA-H19高表达、肺癌组织高分化、淋巴结转移以及TNM高分期是影响肺癌患者预后总生存期的独立危险因素(P<0.05)。结论:Lnc RNA-H19在肺癌组织中的表达增加,Lnc RNA-H19的高表达伴随患者免疫功能的下降和炎症反应因子的增加,提示患者的不良预后。Objective:To investigate the relationship between the expression of long-chain non-coding RNA-H19 and immune function,inflammatory factors and clinical prognosis in lung cancer.Methods:80 patients with lung cancer were enrolled.Lnc RNA-H19 expression in lung cancer and paracancerous tissues was detected by q RT-PCR,serum immunoglobulin was detected by immuno‐turbidimetry,T cell subsets were detected by flow cytometry,inflammatory factors expression was detected by ELISA.The expression of Lnc RNA-H19 and the relationships with clinicopathological features,immune function indexes,inflammatory factors and prognosis were compared.Results:Lnc RNA-H19 expression in lung cancer tissues higher than in paracancerous(P<0.05),and the expression of Lnc RNA-H19 was related to tumor size,differentiation,TNM stage and lymph node metastasis(P<0.05).Lnc RNA-H19 expression in lung cancer tissue was negatively correlated with serum immune function indexes Ig A,Ig G,Ig M,CD3^(+),CD4^(+),CD8^(+)(r<0,P<0.05),and positively correlated with the expression of inflammatory factors IL-1β,IL-6 and TNF-α(r>0,P<0.05).The high expres‐sion of Lnc RNA-H19 in lung cancer tissues was accompanied by the decrease of immune function and the aggravation of inflammatory reaction.The survival rate with high expression of Lnc RNA-H19 was significantly lower than low Lnc RNA-H19 expression(P<0.05).The results of univariate analysis showed that Lnc RNA-H19,tumor differentiation,lymph node metastasis and tumor stage were relat‐ed to overall survival of patients,multivariate analysis showed high Lnc RNA-H19,high differentiation lung cancer,lymph metastasis and high stage were risk factors for overall survival of patients.Conclusion:Expression of Lnc RNA-H19 in lung cancer tissue is in‐creased,and high expression of Lnc RNA-H19 is accompanied by the decrease of immune function and the increase of inflammatory factors,and can indicate patients poor prognosis.

关 键 词：肺癌 长链非编码RNA-H19 免疫功能 炎症反应因子 预后 

分 类 号：R246.5[医药卫生—针灸推拿学]