蓝萼甲素通过cyclinB1/CDK1途径诱导DU145细胞G2/M期阻滞  

Glaucocalyxin A induces G2/M cell cycle arrest through cyclinB1/CDK1 pathway in DU145 cells

作　　者：朱琳琳 徐祉轩 张明明 ZHU Lin-Lin;XU Zhi-Xuan;ZHANG Ming-Ming(Department of Laboratory Medicine,Henan Collaborative Innova-tion Center of Molecular Diagnosis and Laboratory Medicine,Henan Key Laboratory of Immunology and Targeted Therapy,Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院医学检验学院,河南省分子检验与医学检验技术协同创新中心,河南省免疫与靶向药物重点实验室,新乡453003

出　　处：《中国免疫学杂志》2021年第18期2235-2239,共5页Chinese Journal of Immunology

基　　金：河南省科技攻关项目(212102310753)。

摘　　要：目的:研究蓝萼甲素(GLA)调节DU145细胞周期信号通路的表达,探讨GLA阻滞DU145细胞周期的作用机制。方法:不同浓度(1、2.5、5μg/ml)GLA分别刺激培养DU145细胞24 h,CCK8检测细胞增殖;流式检测细胞周期与凋亡;qRT-PCR与Western blot分别检测细胞周期蛋白p21、cyclinB1、CDK1 mRNA与蛋白水平的表达;免疫荧光检测cyclinB1、CDK1在细胞中的定位表达。结果:5μg/ml GLA刺激培养DU145细胞24 h,细胞中p21表达升高,cyclinB1、CDK1表达降低,细胞周期显著阻滞于G2/M期,且细胞凋亡增加。结论:GLA能够通过cyclinB1/CDK1途径诱导DU145细胞周期阻滞于G2/M期,且作用效果与剂量密切相关。Objective:To study the effect of Glaucocalyxin A(GLA)on the expression of cell cycle signaling pathway,and explore the mechanism of GLA blocking the DU145 cell cycle.Methods:DU145 cells were stimulated by 1μg/ml,2.5μg/ml,5μg/ml GLA for 24 hours respectively.Cell proliferation was detected by CCK8.Cell cycle and apoptosis were observed by flow cytometry.P21,cyclinB1 and CDK1 mRNA and protein level were detected by qRT-PCR and Western blot.The location of cyclinB1 and CDK1 were observed by immunofluorescence staining.Results:5μg/ml GLA could increase P21 protein expression obviously but decrease cyclinB1 and CDK1 protein expression conspicuously.In addition,5μg/ml GLA could induce G2/M cell cycle arrest and apoptosis of DU145 cells.Conclusion:GLA may induce G2/M cell cycle arrest through cyclinB1/CDK1 signaling pathway.The effect has relation with the concentration of GLA.

关 键 词：蓝萼甲素 细胞周期蛋白B1 细胞周期蛋白依赖性激酶1 

分 类 号：R392[医药卫生—免疫学]