重灸对失眠模型大鼠下丘脑Orexin-A表达的影响  

Heavy Moxibustion on Expression of Orexin-A in Hypothalamus of Insomnia Model Rats

作　　者：时明伟[1] 蒋西玲 史智君 王孟雨[4] 陈新旺[4] SHI Mingwei;JIANG Xiling;SHI Zhijun;WANG Mengyu;CHEN Xinwang(The Third Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou Henan China 450008;Henan University of Chinese Medicine,Zhengzhou Henan China 450000;The Second People's Hospital of Nanyang City,Nanyang Henan China 473000;Henan University of Chinese Medicine,Zhengzhou Henan China 450008)

机构地区：[1]河南中医药大学第三附属医院,河南郑州450008 [2]南阳市第二人民医院,河南南阳473000 [3]河南省人民医院,河南郑州450008 [4]河南中医药大学,河南郑州450046

出　　处：《中医学报》2021年第10期2208-2212,共5页Acta Chinese Medicine

基　　金：国家自然基金青年基金项目(81704183);河南省科技公关计划项目(172102310014);河南省中医药科学研究专项课题(2017ZY1021,2018ZY2009);河南省高等学校青年骨干教师培养计划(2019GGJS110)。

摘　　要：目的:探讨重灸法对失眠模型大鼠下丘脑Orexin-A表达的影响。方法:将40只SD大鼠随机分为空白组、模型组、重灸组、针刺组和温和灸组,每组8只。除空白组外,其余组大鼠以0.3 g·kg^(-1)的剂量腹腔注射对氯苯丙氨酸溶液,每天1次,连续2 d,建立失眠大鼠模型。建模成功后,重灸组大鼠于背俞穴一次性灸至破溃或化脓,针刺组大鼠于背俞穴进行揿针治疗,温和灸组于背俞穴处进行常规温和灸治疗,空白组、模型组大鼠只固定不做其它处理,干预6天。分别采用免疫组化法和Western Blot法检测下丘脑组织中Orexin-A的表达。结果:与空白组比较,模型组大鼠下丘脑Orexin-A的表达显著升高(P<0.05);与模型组比较,重灸组、针刺组、温和灸组大鼠下丘脑Orexin-A的表达显著降低(P<0.05);与重灸组比较,温和灸组与针刺组大鼠下丘脑Orexin-A的表达显著升高(P<0.05)。结论:重灸法可明显抑制失眠模型大鼠下丘脑Orexin-A的表达。Objective:To observe the effect of heavy moxibustion on expression of Orexin-A in the rat model of insomnia,so as to provide experimental evidence for treating insomnia in clinical treatment.Methods:Forty SD rats were equally randomized into blank group,model group,heavy moxibustion group,acupuncture group and mild moxibustion group(n=8 rat/group).Except for the blank group,rats in the other groups were intraperitoneally injected with p-chlorophenylalanine solution at a dose of 0.3 g/kg,once a day for 2 consecutive days,to establish a rat model of insomnia.After successful modeling,rats in the heavy moxibustion group were moxibustion at the Bei Shu acupoint at one time until they became ulcerated or suppurative;the rats in the acupuncture group were treated with acupuncture at the Backshu point,and the mild moxibustion group was treated with conventional mild moxibustion at the Bei Shu acpoint,The rats in the blank group and model group were only fixed without other treatments,and intervened for 6 days.The expression of Orexin-A in hypothalamic tissue was detected by immune-histochemical method and Western Blot method.Results:Compared with the blank group,the expression of Orexin-A in the hypothalamus of rats in the model group was significantly increased(P<0.05);Compared with the model group,the expression of Orexin-A in the hypothalamus of rats in the heavy moxibustion group,acupuncture group,and mild moxibustion group was significantly reduced(P<0.05).Compared with the heavy moxibustion group,the expression of Orexin-A in the hypothalamus of the mild moxibustion group and the acupuncture group was significantly increased(P<0.05).Conclusion:Heavy moxibustion can significantly inhibit the expression of Orexin-A in the hypothalamus of insomnia model rats.

关 键 词：重灸 失眠 Orexin-A表达 大鼠 

分 类 号：R285.5[医药卫生—中药学]