不同产地山茱萸总皂苷及总环烯醚萜苷的地理变异分析  

Analysis on Geographic Variation of Total Saponins and Total Iridoid Glycosides in Cornus officinalis from Different Habitats

作　　者：安淑静 牛豆 王婷 韩彬凯 王红菊 康杰芳[1] AN Shujing;NIU Dou;WANG Ting;HAN Binkai;WANG Hongju;KANG Jiefang(Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry of Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drug in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, China)

机构地区：[1]陕西师范大学生命科学学院,教育部药用资源与天然药物化学重点实验室,西北濒危药材资源开发国家工程实验室,陕西西安710119

出　　处：《中医药学报》2021年第9期50-55,共6页Acta Chinese Medicine and Pharmacology

基　　金：国家重点研发计划中医药现代化研究重点专项(2017YFC1701302);陕西省重点研发计划重点产业创新链项目(2020ZDLSF05-10,2021ZLDSF04-04)。

摘　　要：目的:对不同产地的山茱萸总皂苷和总环烯醚萜苷进行含量测定,研究不同产地地理因子对其含量影响及之间的相关性,为山茱萸优良产区筛选及质量控制提供相关的理论依据。方法:采用分光光度法和高效液相色谱法对15个产地的山茱萸总皂苷和总环烯醚萜苷含量进行测定,并利用SPSS 19.0软件分别对其含量与产地地理因子进行相关性分析和聚类分析。结果:15个产地的山茱萸总皂苷和总环烯醚萜苷含量变化幅度分别为13.55 mg/g~26.65 mg/g、23.05 mg/g~32.68 mg/g,不同产地之间的活性成分含量差异显著,其中河南西峡样品中总皂苷和浙江淳安样品中总环烯醚萜苷含量高;相关性分析发现总皂苷的含量和产地与海拔呈现极显著的正相关,和经度呈现显著的负相关,环烯醚萜苷含量与环境因子的相关性不显著;聚类分析结果显示,总皂苷与总环烯醚萜苷在东西部产地含量存在明显差异,表现为西高东低的趋势。结论:不同产地的山茱萸总皂苷和总环烯醚萜苷含量不同,存在明显的区域特征、与海拔和经纬度呈现相关性,本研究为山茱萸选择优良的种植产地提供理论支持。Objective:To determine the contents of total saponins and total iridoid glycosides in Cornus officinalis from different habitats,and to study the influence of geographical factors on the contents and the correlation between them,so as to provide theoretical basis for the selection of excellent producing areas and quality control of Cornus officinalis.Methods:The contents of total saponins and total iridoidglycosides in Cornus officinalis collected from 15 different habitats were measured by microplate reader instrument and high performant liquid chromatography respectively,and the relevance analysis and cluster analysis between the contents and geographical factors were carried out by SPASS 19.0 software.Results:The range of contents of total saponins and total iridoid glycosides in Cornus officinalis collected from 15 habitats were 13.55 mg/g~26.65 mg/g and 23.05 mg/g~32.68 mg/g respectively.The samples from Xixia of Henan and Chun’an of Zhejiang had the highest contents of total saponins and total iridoid glycosides.The relevance analysis indicated that the content of total saponins was positively correlated with altitude and negatively with longitude,and the content of total iriodid glycosides had no significant correlation with the geographical factors.Furthermore,the cluster analysis revealed that the contents of total saponins and total iridoid glycosides were significant different between eastern and western habitats,which were higher in the east than the west.Conclusion:There were significant regional characteristics in the contents of total saponins and total iriodid glycosides in Cornus officinalis from different habitats,which showed correlations with altitude and longitude.The study provided theory support for the excellent producing areas for Cornus officinalis.

关 键 词：山茱萸 总环烯醚萜苷 总皂苷 产地 地理变异 

分 类 号：R285[医药卫生—中药学]