近自然管理对橡胶林土壤细菌组成及多样性的影响  

The Effect of Close-to-natural Management on Soil Bacteria in Rubber Plantations

作　　者：李明美 全飞 孙树晴 兰国玉[1,3] 杨川 吴志祥[1,3] LI Ming-mei;QUAN Fei;SUN Shu-qing;LAN Guo-yu;YANG Chuan;WU Zhi-xiang(Ruber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou Hainan 571101,P.R.China;College of Forestry,Hainan University,Haikou Hainan 570228,P.R.China;Danzhou Investigation&Experiment Station of Tropical Crops,Ministry of Agriculture,Danzhou Hainan 571737,P.R.China)

机构地区：[1]中国热带农业科学院橡胶研究所,海南海口571101 [2]海南大学林学院,海南海口570228 [3]农业农村部儋州热带作物科学观测试验站,海南儋州571737

出　　处：《西部林业科学》2021年第5期81-89,共9页Journal of West China Forestry Science

基　　金：海南省重点研发项目社会发展方向项目(ZDYF2019145);国家自然科学基金(31770661);现代农业产业技术体系建设专项资金(CARS-34-ZP3)。

摘　　要：以更好地了解近自然管理方式对于橡胶林土壤细菌的影响为目的,依据自然林核心理论建立一个1 hm 2的橡胶林固定样地进行近自然管理,通过四点采样法进行样品采集,运用土壤农业化学分析方法和基因测序,对土壤理化性质、微生物生物功能、细菌组成等进行实验分析,研究近自然管理后橡胶林土壤细菌群落的变化。结果显示:对橡胶林进行近自然管理后,土壤pH升高,土壤细菌中的酸杆菌门种类减少;橡胶林土壤细菌α多样性并未显著增加,但是在OUT水平上γ多样性(伽玛多样性)显著提高;雨季时近自然管理橡胶林土壤细菌γ多样性高于常规管理,旱季时则相反。研究表明:橡胶林进行近自然管理后土壤pH升高,从而改变了细菌群落的物种组成,细菌多样性提高。其研究结果可为橡胶林近自然管理方式下生物多样性的恢复提供理论依据。In this article,in order to better understand the impact of near-natural management on rubber plantation soil bacteria,a 1 hm 2 rubber plantation fixed sample plot was established for near-natural management based on the core theory of natural forests,and samples were taken through the four-point sampling method,using soil agricultural chemical analysis methods and gene sequencing to conduct experimental analysis on soil physical and chemical properties,microbial biological functions,bacterial composition,etc.,to study the changes in the soil bacterial community of rubber forests after close-to-natural management.The results showed that after the rubber plantation was under natural management,the pH of the soil increased and the number of acid bacteria in the soil bacteria decreased;the α of the soil bacteria in the rubber plantation didn’t increase significantly,but the γ(gamma diversity)increased significantly at the OTU level;theγof soil bacteria in rubber forests under close-to-natural management is higher than that under conventional management in the rainy season,and the opposite is true in the dry season.Conclusion:The soil pH increased after the rubber plantation was under natural management,which changed the species composition of the bacterial community and increased the bacterial diversity.The research results provide a theoretical basis for the restoration of biodiversity under the close-to-natural management of rubber forests.

关 键 词：橡胶林 近自然管理 土壤细菌 细菌组成 多样性 

分 类 号：S714.3[农业科学—林学]