定向栽培连作淮山根际土壤细菌群落的多样性分析  

Analysis of Diversity of Rhizosphere Soil Bacterial Community of Yam in Directional Cultivation in Continuous Cropping

作　　者：周灵芝[1] 劳承英[1] 申章佑[1] 李艳英[1] 黄渝岚 韦本辉[1] 胡泊[1] 周佳[1] ZHOU Ling-zhi;LAO Cheng-ying;SHEN Zhang-you;LI Yan-ying;HUANG Yu-lan;WEI Ben-hui;HU Po;ZHOU Jia(Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China)

机构地区：[1]广西农业科学院经济作物研究所,广西南宁530007

出　　处：《西南农业学报》2021年第8期1601-1607,共7页Southwest China Journal of Agricultural Sciences

基　　金：广西创新驱动发展专项(桂科AA17204045-7,桂科AA20302020);广西农业科学院基本科研业务专项(桂农科2021YT056)。

摘　　要：【目的】分析连作淮山根际土壤细菌群落结构,为解决淮山连作障碍提供参考依据。【方法】以淮山品种桂淮2号为试验材料,设置定向栽培连作淮山(T)和对照(CK)两个处理,采用Illumina MiSeq高通量测序技术结合土壤理化性质,分析连作淮山根际土壤细菌群落多样性及其与土壤理化因子的关系。【结果】基于细菌16S rRNA基因分析,对6个土壤样品测序共得到有效序列230961条,可操作分类单元(OTU)3423个。各样本检测到细菌群落含33门95纲242目395科780属1524种。Venn图分析结果显示,T处理和CK共有细菌OTUs数1688个,T处理特有OTUs数1388个。T处理土壤细菌的Shannon指数、ACE指数和Chao l指数均极显著高于CK(P<0.01,下同)。物种群落组成分析结果表明,放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、绿弯菌门(Chloroflexi)、酸杆菌门(Acidobacteria)和拟杆菌门(Bacteroidetes)是T处理根际土壤细菌的主要优势菌群,占物种群落组成的88.45%。主成分分析结果显示,第一主成分(PC1)和第二主成分(PC2)对细菌群落结构的差异性贡献率分别为81.44%和14.49%,细菌群落结构表现为连作受PC1影响明显。土壤理化性状是影响细菌群落组成的重要因子,与变形菌门、绿弯菌门、拟杆菌门、粘球菌门(Myxococcota)、Patescibacteria、疣微菌门(Verrucomicrobiota)和WPS-2的群落分布显著相关。【结论】淮山连作可提高其根际土壤细菌多样性,其优势细菌群落为放线菌、变形菌、厚壁菌、绿弯菌和酸杆菌;连作淮山土壤pH及水解氮、速效钾和有机质含量的变化是影响其细菌群落组成发生变化的重要因子。【Objective】In order to provide a reference for solving continuous cropping obstacles,the structure of rhizosphere soil bacterial community of yam was discussed in the present paper.【Method】Guihuai 2 was used as material.Two treatments of yam were set up in the way of continuous cropping(T)and control(CK),and Illumina MiSeq high-throughput sequencing technology was used to analyze the diversity of rhizosphere soil bacterial community and its relationship with soil physicochemical factors.【Result】Based on bacterial 16 S rRNA gene analysis,230961 effective sequences and 3423 operational taxon(OTUs)were sequenced from 6 soil samples.33 phyla,95 classes,242 orders,395 families,780 genera and 1524 species were obtained from the bacteria community.Venn diagrams showed that the number of common bacterial OTUs in T treatment and CK groups was 1688,and the specific OUTs number for T treatment was 1388.The Shannon index,Ace index and Chao l index of rhizosphere soil bacteria in yam were all extremely significantly higher than CK(P<0.01).Species community analysis showed that Actinobacteria,Proteobacteria,Firmicutes,Chloroflexi,Acidobacteria and Bacteroidetes were the dominant groups of rhizosphere soil bacteria in yam continuous cropping,totaling 88.45%.Principal component analysis of soil bacterial community showed that the contribution rates of principal component 1(PC1)and principal component 2(PC2)flora structure were 81.44%and 14.49%,respectively,and the bacterial flora structure was influenced by PC1 in the continuous cropping.Soil physical and chemical properties were important factors affecting the bacterial community,which were significantly correlated with the community distributions of Proteobacteria,Chloroflexi,Bacteroidota,Myxococcota,Patescibacteria,Verrucomicrobiota and WPS-2.【Conclusion】Continuous cropping in yam increased the diversity of rhizosphere soil bacteria,and the dominant bacterial communities were Actinobacteria,Proteobacteria,Firmicutes,Chloroflexi and Acidobacteria.pH,available n

关 键 词：淮山 连作 根际土壤 细菌群落 高通量测序 

分 类 号：S154.3[农业科学—土壤学]