Transgenic japonica rice expressing the cry1C gene is resistant to striped stem borers in Northeast China  

作　　者：JIN Yong-mei MA Rui YU Zhi-jing LIN Xiu-feng 

机构地区：[1]Institute of Agricultural Biotechnology,Jilin Academy of Agricultural Sciences/Jilin Provincial Key Laboratory of Agricultural Biotechnology,Changchun 130033,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第11期2837-2848,共12页农业科学学报（英文版）

基　　金：supported by grants from the Jilin Provincial Agricultural Science and Technology Innovation Project in China(CXGC2021TD014);the National Major Project of Breeding for Genetically Modified Organisms in China(2016ZX08001001-001-007)。

摘　　要：Rice production and quality are seriously affected by the lepidopteran pest,striped stem borer(SSB),in Northeast China.In this study,a synthetic cry1 C gene encoding Bacillus thuringiensis(Bt)δ-endotoxin,which is toxic to lepidopteran pest,was transformed into a japonica rice variety(Jigeng 88)in Northeast China by Agrobacterium-mediated transformation.Through molecular detection and the Basta resistance germination assay,a total of 16 single-copy homozygous transgenic lines were obtained from 126 independent transformants expressing cry1 C.Finally,four cry1 C-transgenic lines(JL16,JL23,JL41,and JL42)were selected by evaluation of the Cry1 C protein level,insect-resistance and agronomic traits.The cry1 C-transgenic lines had higher resistance to SSB and higher yield compared with non-transgenic(NT)control plants.T-DNA flanking sequence analysis of the transgenic line JL42 showed that the cry1 C gene was inserted into the intergenic region of chromosome 11,indicating that its insertion may not interfere with the genes near insertion site.In summary,this study developed four cry1 C-transgenic japonica rice lines with high insect resistance and high yield.They can be used as insect-resistant germplasm materials to overcome the problem of rice yield reduction caused by SSB and reduce the use of pesticides in Northeast China.

关 键 词：striped stem borer insect resistance cry1C gene transgenic japonica rice T-DNA flanking sequence 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]