Transcriptome analysis reveals effects of red and blue lightemitting diodes(LEDs)on the growth,chlorophyll fluorescence and endogenous plant hormones of potato(Solanum tuberosum L.)plantlets cultured in vitro  

作　　者：CHEN Li-li WANG Hao-ying GONG Xiao-chen ZENG Zhao-hai XUE Xu-zhang HU Yue-gao 

机构地区：[1]College of Science&Technology,Ningbo University,Cixi 315300,P.R.China [2]College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,P.R.China [3]Beijing Research Center for Intelligent Equipment in Agriculture,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第11期2914-2931,共18页农业科学学报（英文版）

基　　金：funded by the Scientific Research Fund of College of Science&Technology,Ningbo University for the Introduction of High-level Talents,China(RC190006)。

摘　　要：Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.

关 键 词：potato(Solanum tuberosum L.)plantlets in vitro red/blue LEDs light sources RNA-seq chlorophyll fluorescence plant hormone 

分 类 号：S532[农业科学—作物学]