乙醛脱氢酶2对单核细胞-人主动脉内皮细胞黏附的影响及相关机制研究  

Effect of aldehyde dehydrogenase 2 on monocyte-human aortic endothelial cells adhesion and its mechanisms

作　　者：刘宏[1] 吴跃明[1] 陈庆丽[1] 喻秀峰 楼天正[1] Liu Hong;Wu Yueming;Chen Qingli;Yu Xiufeng;Lou Tianzheng(Department of Emergency Medicine,Lishui people's Hospital)

机构地区：[1]丽水市人民医院急诊医学科,丽水323000

出　　处：《重庆医科大学学报》2021年第8期927-932,共6页Journal of Chongqing Medical University

基　　金：丽水市科技计划资助项目(编号:2016zdxk10)。

摘　　要：目的:探讨乙醛脱氢酶2(aldehyde dehydrogenase 2,ALDH2)对单核细胞(human acute monocytic leukemia cells,THP-1)-人主动脉内皮细胞(human aortic endothelial cells,HAECs)黏附的影响及相关机制。方法:将HAECs分为对照(Control)组,ALDH2 NC组,ALDH2组,siRNA-ALDH2 NC组,siRNA-ALDH2-1、-2、-3组,Western blot及real-time PCR检测转染效果,细胞黏附实验检测THP-1-HAECs黏附能力,Western blot及real-time PCR检测核转录因子-κB(nuclear factor-kappa B,NF-κB)转录情况。结果:与Control组、ALDH2 NC组比较,ALDH2组中ALDH2 mRNA及蛋白表达量上调(P<0.05);与Control组、siRNA-ALDH2 NC组比较,siRNA-ALDH2-1、-2、-3组中ALDH2 mRNA及蛋白表达量下调(P<0.05),其中siRNA-ALDH2-2组中ALDH2 m RNA及蛋白表达量最低。与Control组、ALDH2 NC组比较,ALDH2组中THP-1-HAECs黏附能力降低(P<0.05),细胞核中NF-κB表达量下调(P<0.05),细胞质中NF-κB表达量上调(P<0.05),红色荧光减弱(P<0.05);与Control组、siRNAALDH2 NC组比较,siRNA-ALDH2组中THP-1-HAECs黏附能力提高(P<0.05),细胞核中NF-κB表达量上调(P<0.05),细胞质中NF-κB表达量下调(P<0.05),红色荧光增强(P<0.05)。结论:ALDH2通过抑制NF-κB核转录进而抑制THP-1-HAECs黏附,最终缓解动脉粥样硬化。Objective:To investigate the effect of aldehyde dehydrogenase 2(ALDH2)on monocyte(THP-1)-human aortic endothelial cells(HAECs)adhesion and its mechanisms. Methods:HAECs was divided into the Control group,ALDH2 NC group,ALDH2 group,siRNA-ALDH2 NC group,siRNA-ALDH2-1,-2,-3 groups. The expression of ALDH2 protein and mRNA was detected by Western blot and real-time PCR. The adhesive ability between THP-1 and HAECs was detected by cell adhesion assay. The transcription of nuclear factor-kappa B(NF-κB) was detected by Western blot and immunofluorescence. Results:Compared with the Control group and the ALDH2 NC group,the expression of ALDH2 was up-regulated in the ALDH2 group(P<0.05). Compared with the Control group and the siRNA-ALDH2 NC group,the expression of ALDH2 was down-regulated in the siRNA-ALDH2-1,-2,-3 groups(P<0.05),and the expression of ALDH2 was the lowest in the siRNA-ALDH2-2 group. Compared with Control group and ALDH2 NC group,cell adhesive ability,the expression of NF-κB in nucleus and the red fluorescence was decreased(P<0.05),and the expression of NF-κB in cytoplasm increased in ALDH2 group(P<0.05). Compared with Control group and siRNA-ALDH2 NC group,cell adhesive ability,the expression of NF-κB in nucleus and the red fluorescence was increased(P<0.05),the expression of NF-κB in cytoplasm decreased in siRNA-ALDH2 group(P<0.05). Conclusion:ALDH2 could inhibit the adhesion between THP-1-HAECs by inhibiting NF-κB nuclear transcription,and finally alleviate atherosclerosis.

关 键 词：乙醛脱氢酶2 动脉粥样硬化 核转录因子-ΚB 细胞间黏附 

分 类 号：R197[医药卫生—卫生事业管理]